Baculovirus expression system

The baculovirus-insect cells system is widely used to produce recombinant proteins from a wide range of organisms. It makes use of a higher eukaryotic cellular machinery and usually yields overexpressed proteins with proper folding and disulfide bonding.

Cellular localization is usually identical to that of native protein and most post-translational modifications found in higher organisms have been shown to occur usually at the same locations.

The latter cellular processings include signal peptide cleavage, N- and O-glycosilation, phosphorylation, acetylation, amidation, etc.

Protein - Service description

All services include multiple steps from gene to purified protein as described in the table below.

Leadtime is approximately 10-14 weeks.

Description	Resulting Material	Average turnaround (*)
Gene synthesis
-Codon optimization -Gene synthesis -Cloning in standard pUC plasmid -Complete sequencing	Cloned, synthesized and verified gene	2 weeks
Subcloning for expression
-Plasmid prep -Subcloning -Clone selection -Complete sequencing	Plasmid vector with cloned target gene	2 weeks
Pilot expression
-Expression pilot and optimization experiments -Choice of most suitable host -Adjust culture conditions -Determine yields and protein behavior	10-50 µg of semi-purified protein	2-3 weeks
Expression and purification
-Expression -Protein extraction -Protein purification -SDS-PAGE, WESTERN blot	Purified protein	2-3 weeks

(*) Average production time for average difficult project. Actual turn around time varies with individual project features.