Custom genes - Turn around time

Production timelines

The time required for gene synthesis and cloning varies depending on several factors, including sequence complexity, plasmid quantity, and the chosen cloning vector. For most standard services, we offer fast turnaround times to meet your project needs, with details outlined in the table below. If custom cloning into a client-supplied vector is required, please allow an additional 10 days to account for the complexity of this process.

Gene size	Average turnaround time
Gene < 1kb	1 week
Gene 1 to 2 kb	approximatively 2 weeks
Gene 2 to 3 kb	2 to 3 weeks

A sample sequencing trace of a successfully synthesized 1000 bp gene with a poly[AT]50 tail confirms the high fidelity of our gene synthesis service

Some sequences may present unique challenges that extend the turnaround time. Sequences with high GC or AT content (greater than 75%), homopolymer stretches, or repetitive motifs can be difficult to synthesize or clone. In such cases, synthesis times may be longer than standard estimates. For example, sequences prone to forming secondary structures or those with problematic motifs can increase the difficulty and time required for successful cloning.

We pride ourselves on transparent communication, and if any challenges arise during production, you will be promptly informed. Our team works closely with clients to address issues and provide the best solutions, ensuring high-quality results in the shortest possible timeframe.

For more complex projects, such as when the open reading frame (ORF) needs to be cloned into a client-provided vector, we typically require an additional 10 days to complete the process. This extra time ensures that we maintain the highest standards of quality, accuracy, and plasmid integrity, all essential for your downstream applications.