Monoclonal antibodies

Cutting-edge monoclonal antibody development for your research needs

At Genosphere Biotechnologies, we specialize in offering custom monoclonal antibody development services tailored to your specific research requirements. Our commitment to precision and high-quality antibodies ensures that your research objectives are met with the best tools available in the field. Monoclonal antibodies (mAbs) are vital tools in diagnostics, therapeutics, and various biomedical applications, and we take pride in delivering results that match your exact needs in your cutting-edge R&D projects.

Our services are backed by the expertise and technical knowledge of our experienced scientists, who utilize state-of-the-art methodologies and advanced technologies. We leverage innovative techniques to ensure the consistent quality of our products. However, we take our offerings a step further with personalized solutions and unmatched client support.

What are monoclonal antibodies ?

Monoclonal antibodies (mAbs) are highly specialized proteins produced by identical immune cells that are clones of a unique parent cell. These antibodies are engineered to bind specifically to a single antigen, allowing for precise targeting in various applications. Their specificity and uniformity make monoclonal antibodies invaluable tools in both diagnostic and therapeutic contexts, particularly within the realm of precision medicine. They are predominantly produced through hybridoma technology, which involves the fusion of B cells with myeloma cells, or through recombinant DNA techniques, ensuring consistent quality and performance across batches.

Standard package offers

Development of a monoclonal antibody

DESIGNATION	HOST	ANTIGEN	SERVICE	DELIVERABLE	LEAD TIME
Anti-protein monoclonal antibody (you supply your protein antigen)	Mouse	You provide: • Your purified protein • >90% purity • >10 KDa • Amount: 1 to 2 mg	• Immunisation and sera collection • Titer evaluation (ELISA) • Fusion and hybridoma development • ELISA screening and cloning • Isotype determination • Ascites production and purification (2 clones)	• Your purified monoclonal antibody • ELISA result - Titer guaranteed >1:50:000 • Project report	23-26 weeks
Anti-protein monoclonal antibody (we express your protein antigen)	Mouse	Custom protein antigen expression in E. Coli : • DNA sequence design for optimum protein expression • Gene synthesis • Vector construction • Small scale protein expression and purification	• Protein antigen expression and purification • Immunisation and sera collection • Titer evaluation (ELISA) • Fusion and hybridoma development • ELISA screening and cloning • Isotype determination • Ascites production and purification (2 clones)	• Your purified monoclonal antibody • ELISA result - Titer guaranteed >1:50:000 • Project report • Purified recombinant protein	25-28 weeks
Anti-peptide monoclonal antibody (we synthesise the peptide)	Mouse	Custom peptide synthesis: • We prepare synthetic peptide 12-20 aa, with terminal cys for coupling to KLH. • Immunogen: KLH-peptide conjugate	• Target protein analysis and peptide design • Synthesis, purification & conjugation of your peptide (purity >90%) • Immunisation and sera collection • Titer evaluation (ELISA) • Fusion and hybridoma development • ELISA screening and cloning • Isotype determination • Ascites production and purification (2 clones)	• Your purified monoclonal antibody • ELISA result - Titer guaranteed >1:50:000 • Project report • Free peptide, purity >90% with LC & MS analysis	24-28 weeks

Why choose our monoclonal antibody development service?

• Customized antibody production

At Genosphere Biotechnologies, we pride ourselves on delivering fully personalized monoclonal antibody production tailored to the specific needs of research and development. Our approach begins with a thorough understanding of your project requirements, enabling us to create antibodies that are not only specific to your target antigens but also optimized for the intended application. This customization enhances the efficacy of the antibodies for all in vitro studies. The flexibility inherent in our service ensures that we cater to diverse fields such as oncology, infectious disease, and autoimmune research, thereby providing solutions that are highly relevant and impactful.

• Advanced hybridoma technology

Utilizing cutting-edge hybridoma techniques, we ensure the generation of high-affinity, specific monoclonal antibodies. Our hybridoma development process employs the fusion of myeloma cells with antibody-producing B cells, enabling the creation of immortalized cell lines that produce a single type of antibody. This specificity is crucial in applications requiring precise targeting. Our skilled scientists are adept at optimizing hybridoma conditions to enhance yield and specificity, thereby ensuring that the antibodies produced meet the rigorous standards of modern biomedical research.

• High purity & affinity

Our production protocols are designed to achieve the highest standards of purity and affinity. By employing advanced purification techniques, such as Protein A/G chromatography, we eliminate unwanted contaminants, ensuring that the antibodies maintain their structural integrity and functional properties. High purity is vital for reliable experimental results. The antibodies we produce exhibit exceptional binding affinities, which are rigorously quantified through binding assays, ensuring that they meet the specific needs of your application.

• Comprehensive project support

From initial consultation to final delivery, our dedicated team of scientific experts provides guidance and support at every stage of your project. This comprehensive support includes assistance in experimental design, troubleshooting, and protocol optimization, ensuring that you receive not just a product but a collaborative research experience. Our commitment to your success is reflected in our responsive communication and proactive problem-solving, which help streamline your research efforts.

Key features of our monoclonal antibody services

In the rapidly evolving field of biotechnology, the development of monoclonal antibodies (mAbs) plays a crucial role in diagnostics, therapeutics, and research applications. Our comprehensive monoclonal antibody services are designed to address the specific needs of researchers for applications that open the road to future key diagnostics, therapeutics developments. By integrating cutting-edge technology with expert knowledge, we ensure the production of high-quality antibodies that are tailored to your unique requirements. Our services encompass every aspect of mAb development:

1. Antigen design and synthesis

Our team collaborates closely with clients to design and synthesize antigens that are optimized for high specificity and functionality in antibody generation. The choice of antigen—be it a peptide, protein, or other biomolecule—directly influences the efficacy of the resulting monoclonal antibodies. We utilize advanced bioinformatics tools to predict epitopes and enhance antigenicity, ensuring a robust immune response.

2. Hybridoma development

Through our state-of-the-art hybridoma technology, we ensure the production of high-quality monoclonal antibodies. Our experienced scientists meticulously screen and select clones that demonstrate exceptional affinity and specificity for the target antigen. This rigorous selection process is pivotal in generating antibodies that meet the high standards required for cutting-edge research use.

3. Monoclonal antibody purification

Utilizing advanced purification methodologies such as Protein A/G chromatography, we ensure that each batch of monoclonal antibodies achieves the highest levels of purity. This step is essential in eliminating unwanted contaminants and cross-reactive substances, thereby enhancing the reliability of your research results.

4. Characterization & validation

Each monoclonal antibody undergoes a comprehensive validation process before delivery. This includes rigorous testing for specificity, binding affinity, and functionality through a variety of assays, such as ELISA, western blotting, and flow cytometry. These characterizations ensure that our antibodies are ready for immediate use in your research applications.

5. Custom conjugation services

For specialized research applications, we offer custom conjugation services, including the labeling of antibodies with enzymes, fluorescent dyes, or other biomolecules. This enhancement facilitates improved detection methods and expands the utility of your antibodies in diverse experimental settings.

Benefits of working with Genosphere Biotechnologies

At Genosphere Biotechnologies, we understand that successful research hinges on reliable support and efficient processes. Our commitment to excellence is reflected in the range of benefits we offer to our clients in the field of monoclonal antibody development.

• Fast turnaround times

We recognize the critical importance of timely delivery in research settings. Our streamlined processes are designed to expedite project completion while upholding the highest quality standards, ensuring that you meet your research deadlines without compromising on the integrity of your results.

• Cost-effective solutions

Our competitive pricing structure reflects the premium quality of our monoclonal antibody services while remaining accessible to diverse research budgets. We aim to provide cost-effective solutions that empower researchers across various fields without sacrificing quality.

• Expert support team

Our team of scientists is always available for consultations, troubleshooting, and guidance throughout your project lifecycle. This expert support ensures that you can navigate challenges effectively, optimizing the success of your research endeavors.

• Complete intellectual property (IP) ownership

Unlike many providers, we offer complete intellectual property ownership of the developed antibodies, providing you with full control over your research outcomes. This feature is crucial for fostering innovation and ensuring that your discoveries are protected.

Applications of monoclonal antibodies

Monoclonal antibodies serve a vast array of applications across numerous fields in biomedical research and therapeutics. At Genosphere Biotechnologies, we are dedicated to advancing research in critical areas such as :

• Cancer research

Our focus includes the development of therapeutic monoclonal antibodies aimed at cancer immunotherapy and targeted drug delivery systems. These antibodies are pivotal in harnessing the immune system’s potential to combat cancer, providing innovative solutions in oncology.

• Infectious disease research

We specialize in the creation of custom monoclonal antibodies for studying pathogens and immune responses to bacterial, viral, and fungal infections. Our antibodies facilitate the investigation of disease mechanisms and the development of diagnostic tools.

• Diagnostics R&D

Our monoclonal antibodies are integral to the research and development of diagnostic applications, including ELISA and other assay formats. These tools are essential for early disease detection and monitoring, contributing significantly to public health initiatives.

• Autoimmune diseases research

We develop monoclonal antibodies that assist in understanding the mechanisms underlying autoimmune disorders, ultimately aiding in the identification of potential therapeutic targets for these complex diseases.

• Neurological research

Our target-specific monoclonal antibodies play a crucial role in studying neurological disorders, such as Alzheimer’s and Parkinson’s diseases, by allowing researchers to investigate disease pathology and potential therapeutic avenues.

Through our comprehensive monoclonal antibody development services, Genosphere Biotechnologies is committed to supporting your research endeavors with precision, quality, and reliability.

Current trends in monoclonal antibody research

The field of monoclonal antibody (mAb) research is advancing rapidly, with several key trends shaping its future. One notable development is the rise of bispecific antibodies, designed to bind two different epitopes and antigens simultaneously. This innovative approach enhances therapeutic efficacy by engaging multiple pathways, particularly in oncology, improving patient outcomes.

Another significant trend is the emergence of antibody-drug conjugates (ADCs). These biologics link therapeutic monoclonal antibodies to potent cytotoxic agents, delivering drugs directly to target cells and minimizing off-target effects. This targeted delivery system improves therapeutic indices and is currently being evaluated in various clinical trials, showcasing promising results.

Moreover, advancements in CAR-T (chimeric antigen receptor T-cell) therapy highlight the integration of mAbs into cell-based therapies. Utilizing transgenic models, researchers are optimizing CAR-T cells to enhance their ability to recognize and eliminate tumor cells. The synergy between CAR-T technology and mAbs opens new avenues for combination therapies, potentially leading to more durable cancer treatments.

Additionally, affinity maturation techniques are refining mAb specificity and binding strength, improving the reliability of immunoassay development. The application of recombinant technology and B cell hybridoma methods facilitates the rapid production of diverse antibody formats, allowing researchers to explore novel applications.

As these trends continue to evolve, Genosphere Biotechnologies remains dedicated to innovation, leveraging these advancements to enhance monoclonal antibody development and effectively address unmet medical needs.

At Genosphere Biotechnologies, our custom monoclonal antibody development services stand at the forefront of innovation, precision, and client satisfaction. Whether your research requires highly specific antibodies or scalable production options, we have the expertise, technology, and flexibility to deliver results that exceed your expectations. Trust us to provide high affinity, purified, and validated monoclonal antibodies that accelerate your research and drive scientific breakthroughs.

Ready to discuss your custom antibody project?

Our team is here to assist you. Consult us for your next project : ab@genosphere-biotech.com and take the next step in advancing your research with custom antibodies tailored to your needs.