Following polyclonal antibodies production as described above, you may wish to purify your antiserum by chromatography. Genosphere Biotechnologies offers two standard purification services.
Total IgG purification service on Protein A column is recommended for improving signal to noise ratio by eliminating all irrelevant serum proteins and macromolecules. This service includes:
Purification of total IgG from the serum results in a highly pure (>90%) and stabilized polyclonal antibodies preparation.
The second purification service includes affinity chromatography on antigen-bound or peptide-bound support. This approach is recommended when specific IgG are needed in your application, or if further antibodies labeling is intended.
This service includes:
(*) An additional supply of 5 mg of antigen is required for this service.
Affinity chromatography typical elution profile
Affinity chromatography typical elution profile.
ELISA responses of the various fractions for a 15aa anti-peptide antisera.