Genosphere Biotechnologies’ genes are produced in a multistep approach using our proprietaries oligonucleotide design application and macromolecular DNA assembly reactions.
All the sequences are guaranteed to be 100% accurate.
• Codon optimization for heterologous expression: Optimize sequences towards a more favorable codon assortment and thus improving expression in your host.
• Sequence design: Addition or removal of sequence features including N or C purification tags, removal of restriction sites, amino acid changes for structure activities studies, etc.
• Total de novo gene synthesis to your specifications.
• Cloning, screening, amplification and purification of your clone: our standard procedure includes cloning into a pUC-derived plasmid. Optionally, we would clone your gene into any vector that you would provide.
• Complete double-strand sequencing of your gene
Deliverables:
5 µg of purified plasmid DNA, sequencing data and full synthesis report.
With this service, we would introduce any types of mutation into your gene(s) of interest.
♦ Directed single point missense mutations: transitions/transversions – conservative/non-conservative
♦ Simultaneous multiple directed point mutations
♦ Directed in-frame deletions or insertions
♦ 5′- / 3′- in-frame troncations
♦ 5′- / 3′- additions-fusions
Point missense mutations may be introduced in a random fashion within a localized region of your target ORF.
Deliverables include 5 µg of purified individual plasmid DNA and double-stand sequencing data for every variant.
Applications are numerous including:
Basic gene synthesis order includes cloning of your DNA into a standard pUC-derived plasmid. Alternatively, we can clone your DNA sequence into any vector of your choice:
Your gene will thus be ready to use in your experiment. For complex cloning design involving multiple fragments you may want us to perform the experiments for you.
We would require you to provide 5 ug of your purified vector and sequence information.
Basic gene synthesis order includes the delivery of 5 µg of purified cloned plasmid.
Large scale DNA preparation is available at attractive price.
Plasmid Preparation | 50 µg |
Plasmid Preparation | 100 µg |
Plasmid Preparation | 500 µg |
Plasmid Preparation | 1 mg |