Genosphere Biotechnologies

CUSTOM SERVICES FOR RESEARCH
CUSTOMER SERVICES
FOR RESEARCH

Phospho-specific antibodies

This service allows the production of antibodies that will specifically recognize the phosphorylated state of your target protein. The approach includes the synthesis and purification of phosphorylated and non-phosphorylated peptides, immunisation, fabrication of two affinity columns for successive purification. Extensive ELISA on phospho-specific antibodies as well as on the fraction specific to the non-phosphorylated state is performed. We have successfully utilized this method with other post-translational modifications including acetylation and methylation.

H_Phospho_EN_1

ELISA response of the phospho-specific antibodies against (a) the phosphorylated sequence and (b) the non-phosphorylated sequence.

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