Genosphere Biotechnologies

CUSTOM SERVICES FOR RESEARCH
CUSTOMER SERVICES
FOR RESEARCH

Services

Genosphere Biotechnologies makes peptides, antibodies, synthetic genes and recombinant proteins to order for research laboratories in universities, hospitals and industry. Every project is quoted individually and delivered with its quality control report. Our four service areas:

Custom peptide synthesis

Peptides made to order and purified by HPLC, delivered with their analytical profile and mass spectrum. We carry out the usual modifications — cyclic, biotinylated and fluorescent peptides — as well as long biologically active peptides and peptide libraries for screening.

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Custom antibody development

Polyclonal and monoclonal antibodies raised in rabbit, guinea pig, rat, mouse and chicken (IgY from egg yolk). Standard protocol: 4 immunisations over 70 days. The ELISA titre is measured on every project and guaranteed for each antiserum delivered. Anti-peptide, anti-protein and phospho-specific antibodies, affinity-purified on request.

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Custom gene synthesis

De novo gene synthesis from 0.5 kb to 5 kb, with sequence engineering and codon optimisation. Cloning into our standard high-copy pUC plasmid (AmpR) or into the vector of your choice, 5 µg of purified plasmid clone delivered as standard, and double-stranded sequencing data with every gene. Site-directed mutagenesis and large-scale plasmid production are available as well.

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Recombinant protein expression

Expression and purification of recombinant proteins in five systems: the bacterium Escherichia coli, the yeast Pichia pastoris, baculovirus-infected insect cells, HEK or CHO cells, and cell-free in vitro expression. Purity of > 80 % or > 90 % as required, with affinity tags and fluorescent labelling (GFP, RFP, mCherry) on request.

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Need something that is not listed here? Write to us – we look at every request.

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