Escherichia coli is one of the most widely used hosts for the heterologous protein expression. Its genetics are far better characterized than those of any other microorganism.
All services include multiple steps from gene to purified protein as described in the table below.
|Description||Resulting Material||Average turnaround (*)|
|Gene Synthesis Stage|
-Cloning in standard pUC plasmid
|Cloned, synthesized and verified gene||1-2 weeks|
|Subcloning in Expression Vector Stage|
-Subcloning in expression vector
|Expression vector with cloned target gene||1-2 weeks|
|Pilot Expression Stage|
|-Expression vector prep
-Transformation using 3 different strains
-Protein induction and optimzation experiments
-Determine yields and protein behavior
|10-50 µg of semi-purified protein||1-2 weeks|
|Expression and Purification Stage|
|-Expression of 1 to 5 L bacterial culture
-SDS-PAGE, WESTERN blot
| 1-10 mg of purified protein
Purity >80% or >90%
(*) Average production time for average difficult project. Actual turn around time varies with individual project features.