Our company has gained extensive experience in manufacturing research scale difficult and sophisticated peptides. Our synthetic peptides have proven to be generally very stable and to exhibit high biological activity in a wide range of research areas.
Cyclic peptides have drawn considerable interest because of their improved chemical stability and significantly enhanced resistance to in situ enzymatic clearance. The conformation is more rigid then the open-chain analog and may explain in part some of the observed improved biological activities. We routinely prepare cyclic peptides with head to tail ring closure. Directed cys-cys disulfide cyclization is also frequently achieved, and careful monitoring of the reaction ensures 100% cyclization.
Depending upon your application we may prepare biotinylated or fluorescent peptides with various spacers. Aliphatic C3, C6, C12, C18 or hydrophilic polyethylene glycol (PEG)-derived spacers are available.
Our peptides are normally synthesized with unblocked termini i.e. amine and carboxylic acid groups.
However in some instances, one might consider using blocking moieties, or modified ends.
N-acetyl and C-amide closely mimic internal sequences as they have uncharged ends. In addition, these blocking groups improve resistance to enzymatic degradation. It should be noted that solubility is decreased.
Genosphere Biotechnologies provides a wide range of established peptide modification as exemplified below. Many more are available, please don’t hesitate to contact us.
|Unusual Amino acids||Chemical Modifications|
|D-form||Phosphorylation: Tyr, Ser, Thr|
|Fluorescent labeling||Macromolecular Conjugation|
|Fluorescein||Proteins: KLH, BSA, OVA|
|DANSYL||cys-cys disulfide bridge|
|NBD||Head to tail cycle|
|DABCYL||Internal lactam, lactone|