Une sélection de travaux publiés utilisant des peptides, des anticorps, des gènes synthétiques et des protéines recombinantes produits par Genosphere Biotechnologies.

 

 Eclairage sur des travaux de recherches récents

Host-Polarized Cell Growth in Animal Symbionts.

Pende, N., Wang, J., Weber, P.M., Verheul, J., Kuru, E., Rittmann, S.K.R., Leisch, N., VanNieuwenhze, M.S., Brun, Y.V. and Bulgheresi, S., 2018.

Current biology, 28(7), pp.1039-1051.

doi:  10.1016/j.cub.2018.02.028

Keywords:  Cell growth ; cell division ; cell shape ; symbiosis ; peptidoglycan ; MreB ; FtsZ ; Gammaproteobacteria

Abstract:

To determine the fundamentals of cell growth, we must extend cell biological studies to non-model organisms. Here, we investigated the growth modes of the only two rods known to widen instead of elongating, Candidatus Thiosymbion oneisti and Thiosymbion hypermnestrae. These bacteria are attached by one pole to the surface of their respective nematode hosts. By incubating live Ca. T. oneisti and T. hypermnestrae with a peptidoglycan metabolic probe, we observed that the insertion of new cell wall starts at the poles and proceeds inward, concomitantly with FtsZ-based membrane constriction. Remarkably, in Ca. T. hypermnestrae, the proximal, animal-attached pole grows before the distal, free pole, indicating that the peptidoglycan synthesis machinery is host oriented. Immunostaining of the symbionts with an antibody against the actin homolog MreB revealed that it was arranged medially—that is, parallel to the cell long axis—throughout the symbiont life cycle. Given that depolymerization of MreB abolished newly synthesized peptidoglycan insertion and impaired divisome assembly, we conclude that MreB function is required for symbiont widening and division. In conclusion, our data invoke a reassessment of the localization and function of the bacterial actin homolog.

Mitochondrial damage and “plugging” of transport selectively in myelinated, small-diameter axons are major early events in peripheral neuroinflammation.

Sajic, M., Ida, K.K., Canning, R., Gregson, N.A., Duchen, M.R. and Smith, K.J., 2018.

Journal of neuroinflammation, 15(1), p.61.

doi:  10.1186/s12974-018-1094-8

Keywords:  Experimental autoimmune neuritis (EAN) ; Phototoxic damage ; In vivo Confocal imaging ; Mitochondrial ; Small axons ; Pain ; Neuropathy

Abstract:

Small-diameter, myelinated axons are selectively susceptible to dysfunction in several inflammatory PNS and CNS diseases, resulting in pain and degeneration, but the mechanism is not known.We used in vivo confocal microscopy to compare the effects of inflammation in experimental autoimmune neuritis (EAN), a model of Guillain-Barré syndrome (GBS), on mitochondrial function and transport in large- and small-diameter axons. We have compared mitochondrial function and transport in vivo in (i) healthy axons, (ii) axons affected by experimental autoimmune neuritis, and (iii) axons in which mitochondria were focally damaged by laser induced photo-toxicity.Mitochondria affected by inflammation or laser damage became depolarized, fragmented, and immobile. Importantly, the loss of functional mitochondria was accompanied by an increase in the number of mitochondria transported towards, and into, the damaged area, perhaps compensating for loss of ATP and allowing buffering of the likely excessive Ca2+ concentration. In large-diameter axons, healthy mitochondria were found to move into the damaged area bypassing the dysfunctional mitochondria, re-populating the damaged segment of the axon. However, in small-diameter axons, the depolarized mitochondria appeared to "plug" the axon, obstructing, sometimes completely, the incoming (mainly anterograde) transport of mitochondria. Over time (~ 2 h), the transported, functional mitochondria accumulated at the obstruction, and the distal part of the small-diameter axons became depleted of functional mitochondria.The data show that neuroinflammation, in common with photo-toxic damage, induces depolarization and fragmentation of axonal mitochondria, which remain immobile at the site of damage. The damaged, immobile mitochondria can "plug" myelinated, small-diameter axons so that successful mitochondrial transport is prevented, depleting the distal axon of functioning mitochondria. Our observations may explain the selective vulnerability of small-diameter axons to dysfunction and degeneration in a number of neurodegenerative and neuroinflammatory disorders.

 

The neurofilament derived-peptide NFL-TBS. 40-63 enters in-vitro in human neural stem cells and increases their differentiation.

Barreau, K., Montero-Menei, C. and Eyer, J., 2018.

PloS one, 13(8), p.e0201578.

doi:  10.1371/journal.pone.0201578

Keywords:  neurodegenerative diseases ; rat neural stem cells

Abstract:

Regenerative medicine is a promising approach to treat neurodegenerative diseases by replacing degenerating cells like neurons or oligodendrocytes. Targeting human neural stem cells directly in the brain is a big challenge in such a strategy. The neurofilament derived NFL-TBS.40-63 peptide has recently been introduced as a novel tool to target neural stem cells. Previous studies showed that this peptide can be internalized by rat neural stem cells in vitro and in vivo, which coincided with lower proliferation and self-renewal capacity and increase of differentiation. In this study, we analyzed the uptake and potential effects of the NFL-TBS.40-63 peptide on human neural stem cells isolated from human fetuses. We showed that the peptide inhibits proliferation and the ability to produce neurospheres in vitro, which is consistent with an increase in cell adhesion and differentiation. These results confirm that the peptide could be a promising molecule to target and manipulate human neural stem cells and thus could serve as a strategic tool for regenerative medicine.

Phosphatidylserine-liposomes Promote Tolerogenic Features on Dendritic cells in human Type 1 Diabetes by apoptotic Mimicry.

Rodriguez-Fernandez, S., Pujol-Autonell, I., Brianso, F., Perna-Barrull, D., Cano-Sarabia, M., Garcia-Jimeno, S., Villalba, A., Sanchez, A., Aguilera, E., Vazquez, F. and Verdaguer, J., 2018.

Frontiers in immunology, 9, p.253.

doi:  10.3389/fimmu.2018.00253

Abstract:

Type 1 diabetes (T1D) is a metabolic disease caused by the autoimmune destruction of insulin-producing β-cells. With its incidence increasing worldwide, to find a safe approach to permanently cease autoimmunity and allow β-cell recovery has become vital. Relying on the inherent ability of apoptotic cells to induce immunological tolerance, we demonstrated that liposomes mimicking apoptotic β-cells arrested autoimmunity to β-cells and prevented experimental T1D through tolerogenic dendritic cell (DC) generation. These liposomes contained phosphatidylserine (PS)—the main signal of the apoptotic cell membrane—and β-cell autoantigens. To move toward a clinical application, PS-liposomes with optimum size and composition for phagocytosis were loaded with human insulin peptides and tested on DCs from patients with T1D and control age-related subjects. PS accelerated phagocytosis of liposomes with a dynamic typical of apoptotic cell clearance, preserving DCs viability. After PS-liposomes phagocytosis, the expression pattern of molecules involved in efferocytosis, antigen presentation, immunoregulation, and activation in DCs concurred with a tolerogenic functionality, both in patients and control subjects. Furthermore, DCs exposed to PS-liposomes displayed decreased ability to stimulate autologous T cell proliferation. Moreover, transcriptional changes in DCs from patients with T1D after PS-liposomes phagocytosis pointed to an immunoregulatory prolife. Bioinformatics analysis showed 233 differentially expressed genes. Genes involved in antigen presentation were downregulated, whereas genes pertaining to tolerogenic/anti-inflammatory pathways were mostly upregulated. In conclusion, PS-liposomes phagocytosis mimics efferocytosis and leads to phenotypic and functional changes in human DCs, which are accountable for tolerance induction. The herein reported results reinforce the potential of this novel immunotherapy to re-establish immunological tolerance, opening the door to new therapeutic approaches in the field of autoimmunity.


Selected articles

 

A secreted antibacterial neuropeptide shapes the microbiome of Hydra.

Augustin, R. et al.  (2017)  Nature communications, 8(1), p.698.

doi l Europe PMC

Keywords:  Colonization of body epithelial surfaces, sensory neurons, ganglion neurons, ectodermal epithelium, hydra, Cnidaria, neuropeptides, antibacterial, microbiome, NDA-1, reduction of Gram-positive bacteria, Gram-negative Curvibacter sp, β-Proteobacteria, nerve cells, homeostasis, holobiont, biologically active peptides, corals, jellyfishes, polyps, bilateria, metazoans, microbiota, bacteroidetes, antimicrobial

 

 

The leaderless bacteriocin Enterocin K1 is highly potent against Enterococcus faecium: a study on structure, target spectrum and receptor.

Ovchinnikov, K.V. et al.  (2017)  Frontiers in microbiology, 8, p.774.

doi l Europe PMC

Keywords:  enterocin K1, EntK1, enterocin EJ97, EntEJ97, LsbB, bacteriocins, lactococci, Enterococcus faecium, nosocomial multidrug resistant, rseP, stress response, Zn dependent protease, Streptococcus pneumoniae, synthetic ComS peptide

 

 

Ectodomain shedding of CD99 within highly conserved regions is mediated by the metalloprotease meprin β and promotes transendothelial cell migration.

Bedau, T. et al.  (2017)  The FASEB Journal, 31(3), p.1226

doi l Europe PMC

Keywords:  Adhesion molecule CD99 ; Transendothelial migration ; TEM assay ; Metalloprotease meprin β ; γ-secretase ; Intramembrane proteolysis ;  Tissue remodeling ; Inflammation ; Lewis lung carcinoma cells ; Mep1b−/− mice

 

 

High affinity binding of the peptide agonist TIP-39 to the parathyroid hormone 2 (PTH2) receptor requires the hydroxyl group of Tyr-318 on transmembrane helix 5.

Weaver, R.E. et al. (2017). Biochem Pharmacol. , 127, pp. 71-81

doi l Europe PMC

Keywords:  Parathyroid hormone ; PTH ; GPCR ; Agonist ; Receptor ; TIP39 ; Cancer treatment

 

 

Proteomic analyses identify ARH3 as a serine mono-ADP-ribosylhydrolase.

Abplanalp, J. et al.  (2017)  Nature communications, 8(1), p.2055.

doi l Europe PMC

Keywords:  antibodies, histone post-translational modifications, hydrolases, ADP-ribosylation, ARTD1, PARP1, PARG, ARH3, poly-ADP-ribosylation, PARylation, mono-ADP-ribosylation, hydrogen peroxide stress, ADP-ribosylome, nicotinamide adenine dinucleotide

 

 

Altered Protein Interactions of the Endogenous Interactome of PTPIP51 towards MAPK Signaling.

Brobeil, A. et al.  (2017)  Biomolecules, 7(3), p.55.

doi l Europe PMC

Keywords:  Protein–protein interactions, protein tyrosine phosphatase interacting protein 51, PTPIP51, LDC-3, regulation of the endogenous interactome, siRNA, human keratinocytes, HaCaT cells, mitogen activated protein kinase, MAPK, Raf-1, protein scaffold 14-3-3, protein tyrosine phosphatase 1B, PTP1B, dysregulated signal transduction, proliferation, differentiation, apoptosis, basal cell carcinoma, squamous cell carcinoma, acute myeloid leukemia, malignant blasts, EGFR, insulin receptor, nuclear factor κB, transcription factors, NFκB, neurophysin, CGI-99, Nuf2, DGKα, c-Src, mitotic protein complex, calcium homeostasis

 

 

Phytochelatin synthesis in response to Hg uptake in aquatic plants near a chlor-alkali factory.

Turull, M. et al.  (2017)  Chemosphere, 176, pp.74-80.

doi l Europe PMC

Keywords:  Ceratophyllum demersum; Mercury; Myriophyllum spicatum; Phytochelatins

 

 

The human CTF4-orthologue AND-1 interacts with DNA polymerase α/primase via its unique C-terminal HMG box.

Kilkenny, M.L. et al.  (2017)  Open biology, 7(11), p.170217.

doi l Europe PMC

Keywords:  Ctf4-interacting peptide (CIP) motif, DNA replication, replisome, protein–protein interactions, protein–DNA interactions, DNA polymerase, protein hub, Ctf4, chromosome transmission fidelity 4, DNA helicase, Cdc45-MCM-GINS, CTF4 orthologue, AND-1, DNA polymerase α, Pol α / primase, helicase–nuclease Dna2, SepB domain, HMG box, lagging-strand, replication fork, human replisome, Chl1, rDNA, Tof2

 

 

GTL001, a bivalent therapeutic vaccine against human papillomavirus 16 and 18, induces antigen-specific CD8+ T cell responses leading to tumor regression.

Esquerré, M. et al.  (2017)  PloS one, 12(3), e0174038.

doi l Europe PMC

Keywords:  HPV-16 ; Mouse models ; T cells Cytotoxic T cells ; Vaccination and immunization ; Human papillomavirus infection ; Immunologic adjuvants ; Cancer treatment

 

 

A role for the metalloprotease invadolysin in insulin signaling and adipogenesis.

Chang, C.W. et al.  (2017)  Biological Chemistry, 398(3), pp.373-393.

doi l Europe PMC

Keywords: adipogenesis; insulin signaling; lipid droplets; metalloprotease ; Invadolysin ; metazoans ; Drosophila ; glycogen storage ; autophagy

 

 

In Search of Small Molecule Inhibitors Targeting the Flexible CK2 Subunit Interface.

Bestgen, B. et al.  (2017)  Pharmaceuticals, 10(1), p.16.

doi l Europe PMC

Keywords:  cell death; cyclic peptides; protein kinase CK2; protein–protein interaction; subunit interface

 

 

Structural Insight into BLM Recognition by TopBP1.

Sun, L. et al.  (2017)  Structure, 25(10), pp.1582-1588.

doi l Europe PMC

Keywords:  Topoisomerase IIβ binding protein 1, TopBP1, BRCT5, Bloom syndrome helicase, BLM, pSer-binding pocket, DNA damage mediator, MDC1, phosphopeptide interactions, X-ray crystallography, fluorescence polarization, BRCT

 

Serological Analysis of Tuberculosis in Goats by Use of the Enferplex Caprine TB Multiplex Test.

O'Brien. et al.  (2017)  Clinical and Vaccine Immunology, 24(2), e00518-16.

doi l Europe PMC

Keywords:  tuberculosis, antibodies, goats, Mycobacterium avium subsp. paratuberculosis, Mycobacterium bovis, serologic tests, goat diseases diagnosis, veterinary tuberculosis

 

 

Synthetic melanin bound to subunit vaccine antigens significantly enhances CD8+ T-cell responses.

Carpentier, A.F. et al.  (2017)  PloS one, 12(7), p.e0181403.

doi l Europe PMC

Keywords:  melanin, Dopa, tumor, dermatopathic lymphadenopathy, fungal skin disease, melanosomes, memory CD8+ T-cells, TLR9, ovalbumin, TLR, Ephrin-A2, L-Selectin

 

 

 

PipY, a member of the conserved COG0325 family of PLP-binding proteins, expands the cyanobacterial nitrogen regulatory network.

Labella, J.I. et al.  (2017)  Frontiers in microbiology, 8, p.1244.

doi l Europe PMC

Keywords:  Synechococcus elongatus, nitrogen regulation, cyanobacteria, 2-oxoglutarate receptor, 2-OG, global transcriptional regulator, NtcA, co-activator PipX, signal transducer PII, nitrogen and carbon balance, PlmA, PipY, bicistronic operon pipXY, COG0325 proteins, amino/keto acid homeostasis, PLP-related phenotypes, PLP-holoenzymes, synthetic lethality, cysK, autotrophic growth, GS-GOGAT cycle.

 

 

First example of peptides targeting the dimer interface of Leishmania infantum trypanothione reductase with potent in vitro antileishmanial activity.

Ruiz-Santaquiteria, M. et al.  (2017)  European journal of medicinal chemistry, 135, pp.49-59.

doi l Europe PMC

Keywords:  Peptides, Helix stabilization, Protein-protein interactions, Trypanothione reductase, Leishmania infantum, Cell-penetrating peptides, amide-bridged cyclic peptides, lactam peptides, helical turn, Li-TryR

 

 

Mapping in mice the brain regions involved in the anxiolytic-like properties of α-casozepine, a tryptic peptide derived from bovine α s1-casein.

Benoit, S. et al.  (2017)  Journal of Functional Foods, 38, pp.464-473.

doi l Europe PMC

Keywords:  α-Casozepine, α-CZP, bioactive peptide, milk casein, anxiolytic, c-Fos, marker of neuronal activity, diazepam, benzodiazepine, hippocampus, accumbens nucleus, hypothalamus, amygdala, prefrontal cortex, anxiety-inducing situation, tryptic hydrolysate of bovine αs1 casein, GABA, anxiety

 

 

Protoplast swelling and hypocotyl growth depend on different auxin signaling pathways.

Dahlke, R.I. et al.  (2017)  Plant physiology, 175(2), pp.982-994.

doi l Europe PMC

Keywords:  AtABP1 peptide, Arabidopsis thaliana, transport inhibitor response1, auxin signaling f-box protein, TIR1 / AFB, auxin binding protein1, ABP1, hypocotyl growth

 


Cancer-associated mutations in the canonical cleavage site do not influence CD99 shedding by the metalloprotease meprin β but alter cell migration in vitro.

Bedau, T. et al.  (2017)  Oncotarget, 8(33), p.54873.

doi l Europe PMC

Keywords:  peptide, transendothelial cell migration, inflammation, metastasis, adhesion molecule, CD99, extravasation, metalloprotease, meprin β, pro-inflammatory, lung cancer, proteolytic shedding, Src phosphorylation,

 

 

Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis.

O’Brien, D.P. et al.  (2017)  PLoS biology, 15(12), p.e2004486.

doi l Europe PMC

Keywords:  Calmodulin, response to calcium, adenylate cyclase toxin, CyaA, calmodulin binding, cAMP release, AC conformational disorder, calmodulin-activated enzyme, Bordetella pertussis, virulence factor, CaM, hydrogen/deuterium exchange mass spectrometry, disorder-to-order transition, HDX-MS, SAXS, synchrotron radiation circular dichroism, catalytic loop flexibility, long-range allosteric effects,

 

 

Protective effects of βcasofensin, a bioactive peptide from bovine βcasein, against indomethacininduced intestinal lesions in rats.

Bessette, C. et al. (2016)  Molecular nutrition & food research 60(4), 823-33

doi  l  Europe PMC

Keywords:  Milk bioactive peptide ; Mucus ; Intestinal protection ; Functional food ; Inflammatory bowel disease

 

 

Interferon gamma peptidomimetic targeted to interstitial myofibroblasts attenuates renal fibrosis after unilateral ureteral obstruction in mice.

Poosti, F. et al. (2016) Oncotarget, 7(34), pp.54240-54252.

doi  l  Europe PMC

 

 

Identification of a novel cell death-inducing domain reveals that fungal amyloid-controlled programmed cell death is related to necroptosis.
Daskalov, A. et al. (2016) Proceedings of the National Academy of Sciences 113(10), 2720-5.
doi  l  Europe PMC

 

Unraveling flp-11/flp-32 dichotomy in nematodes.
Atkinson, L.E. et al. (2016) International Journal for Parasitology, 46(11), pp.723-736.
doi  l  Europe PMC
Keywords:  FLP-11; FLP-32; FMRFamide-like peptide; Nematode; Neuropeptide; Neuropeptide signalling; flp-11; flp-32

 

TRAIP is a PCNA-binding ubiquitin ligase that protects genome stability after replication stress.
Hoffmann, S. et al. (2016) The Journal of cell biology 212(1), pp.63-75.
doi  l  Europe PMC
Keywords:  Proliferating Cell Nuclear Antigen ; TRAIP protein, human ; Ubiquitin-Protein Ligases

 

(I) Pharmacological profiling of a novel modulator of the α7 nicotinic receptor: Blockade of a toxic acetylcholinesterase-derived peptide increased in Alzheimer brains.
Garcia-Ratés, S. et al. (2016) Neuropharmacology 105, pp.487-499.
doi  l  Europe PMC

 

AMP-activated protein kinase upregulates MAP kinase interacting serine/threonine kinase 1a-dependent phosphorylation of eukaryotic translation initiation factor 4E.
Zhu, X. et al. (2016) Journal of Biological Chemistry, 291(33), pp.17020-17027.
doi  l  Europe PMC

 

Helicobacter pylori exploits human CEACAMs via HopQ for adherence and translocation of CagA.
Königer, V. et al. (2016) Nature Microbiology, 2, p.16188.
doi  l  Europe PMC

 

Nuclear fallout provides a new link between aPKC and polarized cell trafficking.
Calero-Cuenca, F.J. et al. (2016) BMC biology 14(1), p.32
doi  l  Europe PMC
Keywords:  aPKC; Cell polarity; Cell trafficking; Nuclear fallout

 

The corticotropin-releasing factor-like diuretic hormone 44 (DH 44) and kinin neuropeptides modulate desiccation and starvation tolerance in Drosophila melanogaster.

Cannell, E. et al. (2016)  Peptides 80, pp.96-107.

doi  l  Europe PMC
Keywords:  DH44 ; Kinin ; Neuropeptide receptor ; Drosophila melanogaster

 

A cardiac mitochondrial cAMP signaling pathway regulates calcium accumulation, permeability transition and cell death.

Wang, Z. et al. (2016)  Cell death & disease 7(4), p.e2198.

 

Identification of phosphorylation sites regulating sst3 somatostatin receptor trafficking.

Lehmann, A. et al. (2016)  Molecular Endocrinology, 30(6), pp.645-659.

doi Europe PMC
Keywords:  fluorescence-activated cell sorting ; G protein-coupled inwardly rectifying potassium ; G protein-coupled receptor ; GPCR kinase ; hemagglutinin ; human embryonic kidney ; phorbol 12-myristate 13-acetate ; protein phosphatase 1 ; pertussis toxin ; small interfering RNA ; somatostatin-14 ; somatostatin receptor ; tertiapin-Q 

 

Enzymatic properties, evidence for in vivo expression, and intracellular localization of shewasin D, the pepsin homolog from Shewanella denitrificans.

Leal, A.R. et al. (2016)  Scientific reports 6, p.23869

doi  l  Europe PMC

 

Ultrafiltration membrane-based purification of bioconjugated gold nanoparticle dispersions.

Alele, N. et al. (2016)  Separation and Purification Technology 157, pp.120-130.

doi  l  Europe PMC
Keywords:  Ultrafiltration ; Regenerated cellulose membrane ; Nanoparticle ; Bioconjugate ; Colloid purification ; Conjugation efficiency ; Supernatant removal


Interaction between dietary bioactive peptides of short length and bile salts in submicellar or micellar state.

Guerin, J. et al. (2016)   Food chemistry 209, pp.114-122.

doi  l  Europe PMC
Keywords: Bile salts ; CMC ; DLS ; ITC ; Milk whey proteins ; Peptides ; Tryptophan intrinsic fluorescence

 

Hevea brasiliensis prohevein possesses a conserved C-terminal domain with amyloid-like properties in vitro.

Berthelot, K. et al. (2016)  Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1864(4), pp.388-399.

doi  l  Europe PMC
Keywords: Hevea brasiliensis, Plant amyloid, FTIR, Protein aggregation, Latex allergen, Prohevein , Wound-induced protein , Lectin , Chitin binding

 

SAP (E)–A cell-penetrating polyproline helix at lipid interfaces.

Franz, J. et al. (2016)  Biochimica et Biophysica Acta (BBA)-Biomembranes, 1858(9), pp.2028-2034.

doi  l  Europe PMC
Keywords: Cell-penetrating peptide, Peptide-membrane interaction, Polyproline, Sum frequency generation, phosphocholine, cell surface, polypeptide aggregation

 

Peptide aptamer identified by molecular docking targeting translationally controlled tumor protein in leukemia cells.

Kadioglu, O. et al. (2016)  Investigational new drugs, 34(4), 515-21

doi  l  Europe PMC
Keywords: Fortilin, Histamine releasing factor, Leukemia, Molecular docking, Peptide aptamer, TCTP, antineoplastic agents, biomarkers, tumor genetics, leukemia drug therapy, Small interfering RNA

 

Hologne, M. et al. (2016)  Journal of Molecular Biology, 428(22), pp.4544-4558.

doi  l  Europe PMC
Keywords:  endocytosis ; NMR ; deubiquitinating enzymes ; AMSH ; STAM2 

 

Immuno-affinity purification of PglPGIP1, a polygalacturonase-inhibitor protein from pearl millet: studies on its inhibition of fungal polygalacturonases and role in resistance against the downy mildew pathogen.
Prabhu, S. A. et al. (2015) Molecular biology reports 42(6), pp.1123-1138.
doi  l  Europe PMC
Keywords:  Chemical deglycosylation; Confocal immunolocalization; Peptide mass fingerprinting; Plant–pathogen interaction; Quantitative real-time PCR; Two-dimensional gel electrophoresis

 

The structure of the TBCE/TBCB chaperones and α-tubulin complex shows a tubulin dimer dissociation mechanism. 
Serna M et al. (2015)  Journal of Cell Science 128(9), pp. 1824-1834
doi  l  Europe PMC
Keywords: Tubulin; Protein degradation; Chaperones; Folding cofactors; Microtubule

 

EMMPRIN regulates β1 integrin-mediated adhesion through Kindlin-3 in human melanoma cells.
Delyon J et al. (2015) Experimental dermatology, 24(6),  pp. 443-448
doi  l  Europe PMC
Keywords:  EMMPRIN; Kindlin-3; cell adhesion; melanoma; β1 integrin

 

TopBP1 interacts with BLM to maintain genome stability but is dispensable for preventing BLM degradation.
Blackford A N et al. (2015) Mol cell 57, 1133-1141
doi Europe PMC

 

Identification of a Taraxacum brevicorniculatum rubber elongation factor protein that is localized on rubber particles and promotes rubber biosynthesis.
Laibach N et al. (2015) The Plant J 82, 609-620.

 

Use of autoantigen-loaded phosphatidylserine-liposomes to arrest autoimmunity in type 1 diabetes.
Pujol-Autonell I et al. (2015) PloS ONE 10.6, e0127057

 

Influence of Ligands in Metal Nanoparticle Electrophoresis for the Fabrication of Biofunctional Coatings.
Streich C et al. (2015) Appl Surface Sci 348, 92–99

 

Carbon nanotubes and graphene modified screen-printed carbon electrodes as sensitive sensors for the determination of phytochelatins in plants using liquid chromatography with amperometric detection.
Dago A et al. (2015) J Chromatography A 1409, 210–217

 

Klf15 Is Critical for the Development and Differentiation of Drosophila Nephrocytes.
Ivy JR et al. (2015) PLoS ONE 10.8, e0134620

 

New insight into the structure and function of Hfq C-terminus.
Fortas E et al. (2015) Bioscience Rep 35 (2).

Memory and PTPIP51 - A new protein in hippocampus and cerebellum.
Brobeil A et al. (2015) Mol Cell Neurosci 64, 61-73.

Signal Transduction by a Fungal NOD-Like Receptor Based on Propagation of a Prion Amyloid Fold.
Daskalov A et al. (2015) PLoS Biol 13, e1002059

The adhesive properties of the Staphylococcus lugdunensis multifunctional autolysin AtlL and its role in biofilm formation and internalization.
Hussain M et al. (2015) Int J Med Microbiol 305, 129-39

β-Casein(94-123)-derived peptides differently modulate production of mucins in intestinal goblet cells.
Plaisancié P et al. (2015) J Dairy Res 82, 36-46

Full membrane spanning self-assembled monolayers as model systems for UHV-based studies of cell-penetrating peptides.
Franz J et al. (2015) Biointerphases 10(1) , 019009.

Src family kinases interfere with dimerization of STAT5A through a phosphotyrosine-SH2 domain interaction.
Fahrenkamp D et al. (2015) Cell Communication and Signaling 13, 10

Chloride channels in stellate cells are essential for uniquely high secretion rates in neuropeptide-stimulated Drosophila diuresis.
Cabrero P et al. (2014) Proc Natl Acad Sci U S A 111,14301-14306.

Escherichia coli Common Pilus (ECP) Targets Arabinosyl Residues in Plant Cell Walls to Mediate Adhesion to Fresh Produce Plants.
Rossez Y et al. (2014) J Biol Chem 289, 34349.

The role of metalloproteinase ADAM17 in regulating ICOS ligand-mediated humoral immune responses.
Marczynska J et al. (2014) J Immunol 193, 2753-63

Identification of a mitochondrial external NADPH dehydrogenase by overexpression in transgenic Nicotiana sylvestris.
Michalecka AM et al. (2014) Plant J 37, 415-25.

CD31 is a key coinhibitory receptor in the development of immunogenic dendritic cells.
Clement M et al. (2014) Proc Natl Acad Sci U S A 111, E1101-10.

An ancient oxidoreductase making differential use of its cofactors.
D Blüher et al. (2014) Biol Chem 395, 855–869

[The acidic C-terminal tail of the GyrA subunit moderates the DNA supercoiling activity of Bacillus subtilis gyrase.
Lanz MA et al. (2014) J Biol Chem 289, 12275-85

A polymerase chain reaction and enzyme linked immunosorbent assay based approach for diagnosis and differentiation between vaccinated and infected cattle with Mycobacterium bovis.
Sabry M et al. (2014) J Pharm Bioallied Sci 6, 115-21

Study on Aβ34 biology and detection in transgenic mice brains.
Caillava C et al. (2014) Neurobiol Aging 35, 1570-81

Novel cleavable cell-penetrating peptide-drug conjugates: synthesis and characterization.
Lelle M et al. (2014) J Pept Sci 20, 323-33

Effects of synthetic analogues of human opiorphin on rat brain opioid receptors.
Benyhe Z et al. (2014) J Physiol Pharmacol 65, 525-30

Arabidopsis Thaliana AXR4 Recombinant Protein Expression for Polyclonal Antibody Production
Norliza AB et al. (2014) Asian J of Plant Biol 1 , 34-38

Optimising and Evaluating the Characteristics of a Multiple Antigen ELISA for Detection of Mycobacterium bovis Infection in a Badger Vaccine Field Trial.
Aznar I et al. (2014) PloS one 9, e100139.

A novel tumor suppressor function of Kindlin-3 in solid cancer.
Djaafri I et al. (2014) Oncotarget 5, 8970-85.

Suppression of NDA-type alternative mitochondrial NAD(P)H dehydrogenases in arabidopsis thaliana modifies growth and metabolism, but not high light stimulation of mitochondrial electron transport.
Wallström SV et al. (2014) Plant Cell Physiol 55, 881-96

Polylactide block Polypeptide block Polylactide Copolymer Nanoparticles with Tunable Cleavage and Controlled Drug Release.
Dorresteijn R et al. (2014) Adv Funct Mater 24, 4026–4033

Mice deficient for the epidermal dermokine β and γ isoforms display transient cornification defects.
Leclerc EA et al. (2014) J Cell Sci 127, 2862-72

The Expression of the Hepatocyte SLAMF3 (CD229) Receptor Enhances the Hepatitis C Virus Infection.
Cartier F et al. (2014) PLoS ONE 9, e99601

Regulation of the chloroplastic copper chaperone (CCS) and cuprozinc superoxide dismutase (CSD2) by alternative splicing and copper excess in Glycine max.
Sagasti S et al. (2014) Funct Plant Biol 41, 144–155

Dose-dependent inhibition of BACE-1 by the monoterpenoid 2,3,4,4-tetramethyl-5-methylenecyclopent-2-enone in cellular and mouse models of Alzheimer’s disease.
Videira R et al. (2014) J Nat Prod 77, 1275-9

Development of Peptide-Based Lineage-Specific Serology for Chronic Chagas Disease: Geographical and Clinical Distribution of Epitope Recognition.
Bhattacharyya T et al. (2014) PLoS Negl Trop Dis 8, e2892

Interferon gamma peptidomimetic targeted to hepatic stellate cells ameliorates acute and chronic liver fibrosis in vivo
Bansal R et al. (2014) J Controlled Release 179, 18-24

Molecular rules for chemo-and regio-selectivity of Candida antarctica lipase B in peptide acylation reactions
Ferrari F et al. (2014) J Mol Catalysis B: Enzymatic 101, 122-132

Formation Constants of Copper(II) Complexes with Tripeptides Containing Glu, Gly, and His: Potentiometric Measurements and Modeling by Generalized Multiplicative Analysis of Variance.
Khoury RR et al. (2014) Inorg Chem 53, 1278-87

Absence of cell surface expression of human ACE leads to perinatal death.
Michaud A et al. (2014) Hum Mol Genet 23, 1479-91

A high-performance liquid chromatography assay for Dyrk1a, a Down syndrome-associated kinase.
Bui LC et al. (2014) Anal Biochem 449172-8

Surface plasmon resonance analysis of the binding mechanism of pharmacological and peptidic inhibitors to human somatic angiotensin I-converting enzyme.
Zidane F et al. (2013) Biochemistry 52, 8722-31

Signal Peptide Cleavage from GP5 of PRRSV: A Minor Fraction of Molecules Retains the Decoy Epitope, a Presumed Molecular Cause for Viral Persistence.
Thaa B et al. (2013) PLoS One. 8, e65548.

The Tll0287 protein is a hemoprotein associated with the PsbA2-Photosystem II complex in Thermosynechococcus elongatus.
Boussac A et al. (2013) Biochimica et Biophysica Acta 1827, 1174-82

Amino acid transporters expression in acinar cells is changed during acute pancreatitis
Rooman I et al. (2013) Pancreatology 13, 475–485

Calcium-sensing receptor activation increases cell-cell adhesion and β-cell function. Hills CE et al. (2013) Cell Physiol Biochem 30, 575-586.

High-resolution spatio-temporal bioactivity of a novel peptide revealed by optical imaging in rat orbitofrontal cortex in vitro: Possible implications for neurodegenerative diseases.
Badin AS et al. (2013) Neuropharmacology 73, 10-18

Hydrolysis of milk-derived bioactive peptides by cell-associated extracellular peptidases of Streptococcus thermophilus.
Hafeez Z et al. (2013) Appl Microbiol Biotechnol 97, 9787-99

Promoter-dependent expression of the fungal transporter HcPT1.1 under Pi shortage and its spatial localization in ectomycorrhiza.
Garcia K et al. (2013) Fungal Genet Biol 58, 53-61

A necrodane monoterpenoid from Lavandula luisieri essential oil as a cell-permeable inhibitor of BACE-1, the β-secretase in Alzheimer’s disease.
Videira, R et al. (2013) Flavour Fragr. J 28, 380–388

Cathepsin D activity and selectivity in the acidic conditions of a tumor microenvironment: Utilization in the development of a novel Cathepsin D substrate for simultaneous cancer diagnosis and therapy
Achour O et al. (2013) Biochimie 95, 2010–2017

Regulation of the chloroplastic copper chaperone (CCS) and cuprozinc superoxide dismutase (CSD2) by alternative splicing and copper excess in Glycine max
Sagasti S et al. (2013) Functional Plant Biology 41, 144-155

Can bismuth film screen printed carbon electrodes be used to study complexation?
Sosa V et al. (2013) Talanta 107, 356-60

Perturbation of invadolysin disrupts cell migration in zebrafish (Danio rerio)
Vass S et al. (2013) Exp Cell Res 319, 1198–1212.

Glutaminase isoenzymes as key regulators in metabolic and oxidative stress against cancer.
Matés JM et al. (2013) Curr Mol Med 13, 514-34

Opiorphin Secretion Pattern in Healthy Volunteers: Gender Difference and Organ Specificity.
Dufour E et al. (2013) Biochem Anal Biochem 2, 136

Additive Toxicity of β-Amyloid by a Novel Bioactive Peptide In Vitro: Possible Implications for Alzheimer’s Disease.
Garcia-Rates S et al. (2013) PLoS One 8, e54864.

Ataxia with cerebellar lesions in mice expressing chimeric PrP-Dpl protein.
Lemaire-Vieille C et al. (2013) J Neurosci. 33, 1391-1399.

Detection and quantification of staphylococcal enterotoxin A in foods with specific and sensitive polyclonal antibodies
Clarisse T et al. (2013) Food Control 32, 255–261

Measurement and modeling of acid dissociation constants of tri-peptides containing Glu, Gly, and His using potentiometry and generalized multiplicative analysis of variance
Khoury RR et al. (2013) Dalton Trans. 42, 2940-2947

A novel bioactive peptide from yoghurts modulates expression of the gel-forming MUC2 mucin as well as population of goblet cells and Paneth cells along the small intestine.
Plaisancié P et al. (2013) J Nutr Biochem. 24, 213-21

flp-32 Ligand/Receptor Silencing Phenocopy Faster Plant Pathogenic Nematodes.
Atkinson LE et al. (2013) PLoS Pathog 9, e1003169

GSK3-mediated VDAC phosphorylation controls outer mitochondrial membrane permeability during lipid accumulation.
Martel C et al. (2013) Hepatology 57, 93-102.

Expression and localization of glutamate-gated chloride channel variants in honeybee brain (Apis mellifera).
Demares F et al. (2013) Insect Biochem Mol Biol. 43, 115-24

The metalloprotease meprin β generates amino terminal truncated Aβ-peptide species.
Bien J et al. (2012) J Biol Chem. 287, 33304-13.

Competitive binding of UBPY and ubiquitin to the STAM2 SH3 domain revealed by NMR.
Lange A et al. (2012) FEBS Lett. 586, 3379-84

Staphylococcus aureus Panton-Valentine leukocidin induces an inflammatory response in human phagocytes via the NLRP3 inflammasome.
Holzinger D et al. (2012) J Leukoc Biol. 92, 1069-81.

ANT-VDAC1 interaction is direct and depends on ANT isoform conformation in vitro.
Allouche M et al. (2012) Biochem Biophys Res Commun. 429, 12-17

Dissecting mannose 6-phosphate-insulin-like growth factor 2 receptor complexes that control activation and uptake of plasminogen in cells.
Leksa V et al. (2012) J Biol Chem. 287, 22450-62

PTPIP51 in protein interactions: regulation and in situ interacting partners.
Brobeil A et al. (2012) Cell Biochem Biophys. 63, 211-22.

NMR structure of hsp12, a protein induced by and required for dietary restriction-induced lifespan extension in yeast.
Herbert AP et al. (2012) PLoS One. 7, e41975

Investigations of the supramolecular structure of individual diphenylalanine nano- and microtubes by polarized Raman microspectroscopy.
Lekprasert B et al. (2012) Biomacromolecules. 13, 2181-7

Mammalian glutaminase Gls2 gene encodes two functional alternative transcripts by a surrogate promoter usage mechanism.
Martín-Rufián M et al. (2012) PLoS One. 7, e38380

Identification, localization and function of glutamate-gated chloride channel receptors in the honeybee brain.
El Hassani AK et al. (2012) Eur J Neurosci. 36, 2409-20

Opiorphin highly improves the specific binding and affinity of MERF and MEGY to rat brain opioid receptors.
Tóth F et al. (2012) Regul Pept. 178, 71-5

A CD31-derived peptide prevents angiotensin II-induced atherosclerosis progression and aneurysm formation.
Fornasa G et al. (2012) Cardiovasc Res. 94, 30-7

Targeted and intracellular triggered delivery of therapeutics to cancer cells and the tumor microenvironment: impact on the treatment of breast cancer.
Moura V et al. (2012) Breast Cancer Res Treat. 133, 61-73

Chlamydia co-opts the rod shape-determining proteins MreB and Pbp2 for cell division.
Ouellette SP et al. (2012) Mol Microbiol. 85, 164-78

Marginal zone B cells regulate antigen-specific T cell responses during infection.
Bankoti R et al. (2012) J Immunol. 188, 3961-71

Functional and conformational changes in the aspartic protease cardosin A induced by TFE.
Fraga AS et al. (2012) Int J Biol Macromol. 50, 323-30

Increased expression of VDAC1 sensitizes carcinoma cells to apoptosis induced by DNA cross-linking agents.
Sharaf El Dein O et al. (2012) Biochem Pharmacol. 83, 1172-82

Mechanism and function of Drosophila capa GPCR: a desiccation stress-responsive receptor with functional homology to human neuromedinU receptor.
Terhzaz S et al. (2012) PLoS One. 7, e29897

Calcium-induced folding of intrinsically disordered repeat-in-toxin (RTX) motifs via changes of protein charges and oligomerization states.
Sotomayor-Pérez AC et al. (2011) J Biol Chem. 286, 16997-7004

Loss-of-function point mutations associated with renal tubular dysgenesis provide insights about renin function and cellular trafficking.
Michaud A et al. (2011) Hum Mol Genet. 20, 301-11

Expression of Drosophila Cabut during early embryogenesis, dorsal closure and nervous system development.
Belacortu Y et al. (2011) Gene Expr Patterns. 11, 190-201.

Use of a multiplex enzyme-linked immunosorbent assay to detect a subpopulation of Mycobacterium bovis-infected animals deemed negative or inconclusive by the single intradermal comparative tuberculin skin test.
Whelan C et al. (2011) J Vet Diagn Invest. 23, 499-503.

Peptide-regulated gene depletion system developed for use in Streptococcus pneumoniae.
Berg KH et al. (2011) J Bacteriol. 193, 5207-15.

Distinct expression, localization and function of two Rab7 proteins encoded by paralogous genes in a free-living model eukaryote.
Osińska M et al. (2011) Acta Biochim Pol. 58, 597-607.

Plk5, a Polo-box domain-only protein with specific roles in neuron differentiation and glioblastoma suppression.
de Cárcer G et al. (2011) Mol Cell Biol. 31, 1225-39.

Expression and Localization of Aquaporin 1a in the Sea-Bass (Dicentrarchus labrax) during Ontogeny.
Giffard-Mena I et al. (2011) Front Physiol. 2, 34

Characterization of the recombinant copper chaperone (CCS) from the plant Glycine (G.) max.
Sagasti S et al. (2011) Metallomics 3, 169-75.

Schistosome I/Lamides—a new family of bioactive helminth neuropeptides.
McVeigh P et al. (2011) Int J Parasitol. 8, 905-13.

Shewasin A, an active pepsin homolog from the bacterium Shewanella amazonensis.
Simões et al. (2011) FEBS J. 278, 3177-86.

Sequence variability of the pattern recognition receptor Mermaid mediates specificity of marine nematode symbioses.
Bulgheresi S et al. (2011) ISME J. 5, 986-98.

Soluble M6P/IGF2R Released by TACE Controls Angiogenesis via Blocking Plasminogen Activation.
Leksa V et al. (2011) Circ Res. 108, 676-85.

Deletion of UCP2 in iNOS deficient mice reduces the severity of the disease during experimental autoimmune encephalomyelitis.
Aheng C et al. (2011) PLoS ONE 6, e22841.

Peptides derived from the bifunctional kinase/RNase enzyme IRE1alpha modulate IRE1alpha activity and protect cells from endoplasmic reticulum stress.
Bouchecareilh M et al. (2011) FASEB J. 25, 3115-29.

Plasmacytoid dendritic cells play a major role in apoptotic leukocyte-induced immune modulation.
Bonnefoy F et al. (2011) J Immunol. 186, 5696-705.

Secretory leukocyte protease inhibitor (SLPI) is, like its homologue trappin-2 (Pre-Elafin), a transglutaminase substrate.
Baranger K et al. (2011) PLoS ONE 6, e20976.

Transcriptome and metabolite profiling show that APETALA2a is a major regulator of tomato fruit ripening.
Karlova R et al. (2011) Plant Cell. 23, 923-41.

Inhibition of Nuclear Translocation of Calcineurin Suppresses T-Cell Activation and Prevents Acute Rejection of Donor Hearts.
Panther F et al. (2011) Transplantation 91, 597-604.

Use of allostery to identify inhibitors of calmodulin-induced activation of Bacillus anthracis edema factor.
Laine E et al. (2010) Proc Natl Acad Sci U S A. 107, 11277-82.

Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y˙) involved in ribonucleotide reduction.
Stolle P et al. (2010) FEBS J. 277, 4849-62

Serine protease-mediated host invasion by the parasitic nematode Steinernema carpocapsae.
Toubarro D et al. (2010) J Biol Chem. 285, 30666-75.

A combined zinc/cadmium sensor and zinc/cadmium export regulator in a heavy metal pump.
Baekgaard L et al. (2010) J Biol Chem. 285, 31243-52

Calcium-induced folding and stabilization of the intrinsically disordered RTX domain of the CyaA toxin.
Chenal A et al. (2010) Biophys J. 99, 3744-53

The IkappaB kinase inhibitor nuclear factor-kappaB essential modulator-binding domain peptide for inhibition of injury-induced neointimal formation.
Grassia G et al. (2010) Arterioscler Thromb Vasc Biol. 30, 2458-66

Nondestructive Raman and atomic force microscopy measurement of molecular structure for individual diphenylalanine nanotubes.
Lekprasert B et al. (2010) Opt Lett. 35, 4193-5.

Mislocalization of mitochondria and compromised renal function and oxidative stress resistance in Drosophila SesB mutants.
Terhzaz S et al. (2010) Physiol Genomics 41, 33-41.

Systemically active human opiorphin is a potent yet non-addictive analgesic without drug tolerance effects.
Rougeot C et al. (2010) J Physiol Pharmacol. 61, 483-90.

Sensitive liquid chromatography/tandem mass spectrometry assay for absolute quantification of ITIH4-derived putative biomarker peptides in clinical serum samples.
van den Broek I et al. (2010) Rapid Commun Mass Spectrom. 24, 1842-50.

Salt stress causes peroxisome proliferation, but inducing peroxisome proliferation does not improve NaCl tolerance in Arabidopsis thaliana.
Mitsuya S et al. (2010) PLoS One. 5, e9408.

Integrin activation by Fam38A uses a novel mechanism of R-Ras targeting to the endoplasmic reticulum.
McHugh BJ et al. (2010) J Cell Sci. 123, 51-61.

Characterization of the regions involved in the calcium-induced folding of the intrinsically disordered RTX motifs from the bordetella pertussis adenylate cyclase toxin.
Sotomayor Pérez AC et al. (2010) J Mol Biol. 397, 534-49

Evaluation of a technique to identify acetylcholinesterase C-terminal peptides in human serum samples.
Halliday AC et al. (2010) Chem Biol Interact. 187, 110-114

ICAM-4 and CD36 are implicated in the abnormal adhesiveness to endothelium of sickle cell SAD mouse erythrocytes.
Trinh-Trang-Tan et al. (2010) Haematologica 95, 730-7.

TCR stimulation drives cleavage and shedding of the ITIM receptor CD31.
Fornasa G et al. (2010) J Immunol. 184, 5485-92

The fourth isoform of the adenine nucleotide translocator inhibits mitochondrial apoptosis in cancer cells.
Gallerne C et al. (2010) Int J Biochem Cell Biol. 42, 623-9

Purification and Characterization of Avian beta-Defensin 11 (AvBD11): an Antimicrobial Peptide of the Hen Egg.
Hervé-Grépinet V et al. (2010) Antimicrob Agents Chemother. 54, 4401-9

Control of T cell reactivation by regulatory Qa-1-restricted CD8+ T cells.
Varthaman A et al. (2010) J Immunol. 184, 6585-91

Characterization of SUN-domain proteins at the higher plant nuclear envelope.
Graumann K et al. (2010) Plant J. 61, 134-44.

Human opiorphin is a naturally occurring antidepressant acting selectively on enkephalin-dependent delta-opioid pathways.
Javelot H et al. (2010) J Physiol Pharmacol. 61, 355-62.

The major determinant of exendin-4/glucagon-like peptide 1 differential affinity at the rat glucagon-like peptide 1 receptor N-terminal domain is a hydrogen bond from SER-32 of exendin-4
Mann RJ et al. (2010) Br J Pharmacol 160, 1973-84.

Pneumococcal CbpD is a murein hydrolase that requires a dual cell envelope binding specificity to kill target cells during fratricide.
Eldholm V et al. (2010) Mol Microbiol. 76, 905-17.

Antibody to CCDC104 is associated with a paraneoplastic antibody to CDR2 (anti-Yo).
Totland C et al. (2010) Cancer Immunol Immunother. 59, 231-237

Calmodulin-Induced Conformational and Hydrodynamic Changes in the Catalytic Domain of Bordetella pertussis Adenylate Cyclase Toxin.
Karst JC et al. (2010) Biochemistry 49, 318–328

Evaluation of the minimal replication time of Cauliflower mosaic virus in different hosts.
Khelifaa M et al. (2010) Virology 396, 238-245

GD3–7-aldehyde is an apoptosis inducer and interacts with adenine nucleotide translocase.
Brenner C et al. (2010) Biochem Biophys Res Commun. 391, 248-253

Generation and characterisation of Rhd and Rhag null mice.
Goossens D et al. (2010) Br J Haematol. 148, 161-172

Staphylococcus aureus panton-valentine leukocidin is a very potent cytotoxic factor for human neutrophils.
Löffler B et al. (2010) PLoS Pathog. 6, e715

The beta-barrel outer membrane protein assembly complex of Neisseria meningitidis.
Volokhina EB et al. (2009) J Bacteriol. 191, 7074-85

NEMO-binding domain peptide inhibits proliferation of human melanoma cells.
Ianaro A et al. (2009) Cancer Lett. 274, 331-336

The conserved metalloprotease invadolysin localizes to the surface of lipid droplets.
Cobbe Net al. (2010) J Cell Sci. 122, 3414-23.

Discovery of multiple neuropeptide families in the phylum Platyhelminthes.
McVeigh P et al. (2009) Int J Parasitol. 39, 1243-52.

Salmonella serovar specific upregulation of porcine defensins 1 and 2 in a jejunal epithelial cell line.
Veldhuizen EJ et al. (2009) Vet Microbiol. 136, 69-75

Endocytosis controls glutamate-induced nuclear accumulation of ERK.
Trifilieff P et al. (2009) Mol Cell Neurosci. 41, 325-336

Upregulation of alpha7 Nicotinic Receptors by Acetylcholinesterase C-Terminal Peptides.
Bond CE et al. (2009) PLoS ONE. 4, e4846

Identification and characterization of two novel truncated but functional isoforms of the somatostatin receptor subtype 5 differentially present in pituitary tumors.
Durán-Prado M et al. (2009) J Clin Endocrinol Metab. 94, 2634-43

Production of an agonist-like monoclonal antibody to the human A2A receptor of adenosine for clinical use.
By Y et al. (2009) Mol Immunol. 46, 400-405

Vibrational Analysis of Amino Acids and Short Peptides in Aqueous Media. V. The Effect of the Disulfide Bridge on the Structural Features of the Peptide Hormone Somatostatin-14.
Hernandez B et al. (2009) J Phys Chem B. 113, 12796–12803

Downregulation of Platelet Responsiveness Upon Contact With LDL by the Protein-Tyrosine Phosphatases SHP-1 and SHP-2.
Korporaal S et al. (2009) Arterioscler Thromb Vasc Biol. 29, 372-379

Human endothelial cells of the placental barrier efficiently deliver cholesterol to the fetal circulation via ABCA1 and ABCG1.
Stefulj J et al. (2009) Circ Res. 104, 600-608

Humoral and cellular immune responses to myelin protein peptides in chronic inflammatory demyelinating polyradiculoneuropathy.
Sanvito L et al. (2009) J Neurol Neurosurg Psychiatry 80, 333-338.

Proteomics of Multigenic Families from Species Underrepresented in Databases: The Case of Loquat (Eriobotrya japonica Lindl.) Polyphenol Oxidases.
Sells-Marchart S et al. (2008) J Proteome Res. 7, 4095–4106

Multiplex Immunoassay for Serological Diagnosis of Mycobacterium bovis Infection in Cattle.
Whelan C et al.(2008) Clin Vaccine Immunol. 15, 1834-1838.

The Leishmania ARL-1 and Golgi Traffic.
Sahin A et al.(2008) PLoS ONE. 3, 1620.

Olive pollen profilin (Ole e 2 allergen) co-localizes with highly active areas of the actin cytoskeleton and is released to the culture medium during in vitro pollen germination.
Morales S et al.(2008) J Microsc. 231, 332-41.

Chronic sensorimotor polyradiculopathy with antibodies to P2: an electrophysiological and immunoproteomic analysis.
Rojas-Garcia R et al.(2008) Muscle Nerve 38, 933-938.

Liquid chromatography/tandem mass spectrometric method for the quantification of eight proteolytic fragments of ITIH4 with biomarker potential in human plasma and serum.
van den Broek I et al.(2008) Rapid Commun Mass Spectrum 22, 2915-2928.

Dedicated Metallochaperone Connects Apoenzyme and Molybdenum Cofactor Biosynthesis Components.
Genest O et al.(2008) J Biol Chem. 283, 21433-21440.

Phosphorylation of the C subunit (p66) of human DNA polymerase delta.
Lemmens L et al. (2008) Biochem Biophys Res Commun. 367, 264-267.

Selective enhancement of the activity of C-terminally truncated, but not intact, acetylcholinesterase.
Zimmermann, M et al. (2008) J Neurochem. 104, 221-232.

Porcine beta-defensin 2 displays broad antimicrobial activity against pathogenic intestinal bacteria.
Veldhuizen EJ et al. (2008) Mol Immunol. 45, 386-94.

Pax3 regulates Wnt1 expression via a conserved binding site in the 5’ proximal promoter.
Fenby BT et al. (2008) Biochim Biophys Acta. 1779, 115-21.

Immune responses to myelin proteins in Guillain-Barré syndrome.
Makowska A et al.(2008) J Neurol Neurosurg Psychiatry 79, 664-671.

Hydrolysis of caprine and ovine milk proteins, brought about by aspartic peptidases from Silybum marianum flowers.
Vairo Cavallia S et al. (2008) J Food Chem. 106, 997-1003.

The C-terminus of the y2 chain but not of b3 chain of laminin-332 is indirectly but indispensably necessary for integrin-mediated cell reactions.
Navdaev A et al. (2008) Experim Cell Res. 314, 489-497.

Structural and functional studies of a family of Dictyostelium discoideum developmentally regulated, prestalk genes coding for small proteins.
Vicente J J et al. (2008) BMC Microbiol. 8, 1.

Basis of Arginine Sensitivity of Microbial N-Acetyl-l-Glutamate Kinases: Mutagenesis and Protein Engineering Study with the Pseudomonas aeruginosa and Escherichia coli Enzymes.
Fernández-Murga M L et al. (2008) J Bacteriol. 190, 3018–3025.

Reduced circulating CD4+CD25+ cell populations in Guillain–Barré syndrome.
Pritchard J et al.(2007) J Neuroimmunol. 183, 232-238.

Stress-dependent relocalization of translationally primed mRNPs to cytoplasmic granules that are kinetically and spatially distinct from P-bodies.
Hoyle NP et al.(2007) J Cell Biol. 179, 65-74.

Characterization of recombinant CDR1, an Arabidopsis aspartic proteinase involved in disease resistance.
Simões I et al. (2007) J Biol Chem. 282, 31358-65.

A TAT-DEF-Elk-1 peptide regulates the cytonuclear trafficking of Elk-1 and controls cytoskeleton dynamics.
Lavaur J et al. (2007) J. Neurosci. 27, 14448-58.

The regulatory factor SipA provides a link between NblS and NblR signal transduction pathways in the cyanobacterium Synechococcus sp. PCC 7942.
Salinas P et al. (2007) Mol Microbiol. 66, 1607-19.

The ß-defensin Gallinacin-6 is expressed in the chicken digestive tract and has antimicrobial activity against food-borne Pathogens.
van Dijk A et al. (2007) Antimicrob Agents Chemother. 51, 912-22.

Molecular and structural basis of polo-like kinase 1 substrate recognition: Implications in centrosomal localization.
García-Álvarez B et al. (2007) Proc Natl Acad Sci USA. 104, 3107-12.

The Major Secreted Cathepsin L1 Protease of the Liver Fluke, Fasciola hepatica:. A leu-12 to pro-12 replacement in the non-conserved C-terminal region of the prosegment prevents complete enzyme autoactivation and allows definition of the molecular events in prosegment removal.
Colin M et al. (2007) J. Biol. Chem. 282, 16532-16543.

Steam-girdling of barley (Hordeum vulgare) leaves leads to carbohydrate accumulation and accelerated leaf senescence, facilitating transcriptomic analysis of senescence-associated genes .
David L. et al. (2007) New Phytologist 176, 56–69.

Identification and subcellular localization of the soybean copper P1B-ATPase GmHMA8 transporter .
María Bernal, Pilar S. et al. (2007) J Struct Biol. 158, 46-58.

An acetylation/deacetylation-SUMOylation switch through a phylogenetically conserved psiKXEP motif in the tumor suppressor HIC1 regulates transcriptional repression activity.
Stankovic-Valentin N et al. (2007) Mol Cell Biol. 27, 2661-75.

Metabolic regulation of SIRT1 transcription via a HIC1:CtBP corepressor complex. .
Zhang Q et al. (2007) Proc Natl Acad Sci U S A. 104, 829-33.

Inhibition of Nuclear Import of Calcineurin Prevents Myocardial Hypertrophy.
Hallhuber M et al. (2007) Circulation Res. 99, 626-35.

The SzA mutations of the B subunit of the Drosophila vacuolar H+ ATPase identify conserved residues essential for function in fly and yeast.
Du J et al. (2007) J Cell Sci. 119, 2542-51.

Pheromone-induced expression of recombinant proteins in Streptococcus thermophilus.
Blomqvist T et al. (2007) Arch Microbiology. 186, 465-73.

Basolateral aromatic amino acid transporter TAT1 (Slc16a10) functions as an efflux pathway.
Ramadan T et al. (2006) J Cell Physiol. 206, 771-9.

The lipidation status of acute-phase protein serum amyloid A determines cholesterol mobilization via scavenger receptor class B, type I.
Marsche G et al. (2006) Biochem J. 402, 117-24.

Comparison of recombinant adenovirus and synthetic peptide for DC-based melanoma vaccination.
Steitz J et al. (2006) Cancer Gene Ther. 13, 318-25.

Simvastatin promotes Th2-type responses through the induction of the chitinase family member Ym1 in dendritic cells.
Arora M et al. (2006) Proc Natl Acad Sci U S A. 103, 7777-82.

Antithrombotic effect of the type III collagen-related octapeptide (KOGEOGPK) in the mouse.
Maurice P et al. (2006) Vascul Pharmacol. 44, 42-49

The lipidation status of acute-phase protein serum amyloid A determines cholesterol mobilization via scavenger receptor class B, type I.
Marsche G et al. (2006) Biochem J 402, 117-24.

A L225A substitution in the human tumour suppressor HIC1 abolishes its interaction with the corepressor CtBP.
Stankovic-Valentin N et al. (2006) FEBS J. 273, 2879-90.

Evaluation of DNA vaccination with recombinant adenoviruses using bioluminescence imaging of antigen expression: impact of application routes and delivery with dendritic cells.
Schweichel D et al. (2006) J Gene Med. 8, 1243-50.

Diverse mitotic and interphase functions of condensins in Drosophila.
Cobbe N et al. (2006) Genetics 172, 991-1008.

Ventricular pre-excitation and cardiac hypertrophy mimicking hypertrophic cardiomyopathy in a Turkish family with a novel PRKAG2 mutation.
Bayrak F et al. (2006) Eur J Heart Fail. 8, 712-5.

A novel role for a Drosophila homologue of cGMP-specific phosphodiesterase in the active transport of cGMP.
Day JP et al. (2006) Biochem J. 393, 481-8.

A hydrophobic patch in the competence-stimulating Peptide, a pneumococcal competence pheromone, is essential for specificity and biological activity.
Johnsborg O et al. (2006) J Bacteriol. 188, 1744-9.

A membrane-destabilizing peptide in capsid protein L2 is required for egress of papillomavirus genomes from endosomes.
Kamper N et al. (2006) J Virol. 80, 759-68.

Evaluation of genetic melanoma vaccines in cdk4-mutant mice provides evidence for immunological tolerance against authochthonous melanomas in the skin.
Steitz J et al. (2006) Int J Cancer. 118, 373-80.

Identification of new Golgi complex specific proteins by direct organelle proteomic analysis.
Takatalo M S et al. (2006) Proteomics 6, 3502-08.

Deletion of the V1/V2 region does not increase the accessibility of the V3 region of recombinant gp125.
Sourial S et al. (2006) Curr HIV Res. 4, 229-37.

Novel subcellular locations and functions for secretory pathway Ca2+/Mn2+-ATPases.
Southall TD et al. (2006) Physiol Genomics. 26, 35-45.

Crystallization and preliminary X-ray diffraction studies on the human Plk1 Polo-box domain in complex with an unphosphorylated and a phosphorylated target peptide from Cdc25C.
García-Álvarez B et al. (2006) Acta Cryst. 62, 372-75.

Therapeutic Efficacy of Antigen-Specific Vaccination and Toll-Like Receptor Stimulation.
Tormo D et al. (2006) Cancer Res. 66, 5427-35.

Differential gel electrophoresis and transgenic mitochondrial calcium reporters demonstrate spatiotemporal filtering in calcium control of mitochondria.
Terhzaz S et al. (2006) J Biol Chem. 281, 18849-58.

A hypervariable N-terminal region of Yersinia LcrV determines Toll-like receptor 2-mediated IL-10 induction and mouse virulence.
Sing A et al. (2005) Proc Natl Acad Sci USA. 102, 16049-54.

Three Peroxisome Proliferator-Activated Receptor Isotypes from Each of Two Species of Marine Fish.
Leaver MJ et al. (2005) Endocrinol. 146, 3150-3162.

AtPEX2 and AtPEX10 are targeted to peroxisomes independently of known endoplasmic reticulum trafficking routes.
Sparkes IA et al. (2005) Plant Physiol. 139, 690-700.

A novel diuretic hormone receptor in Drosophila: evidence for conservation of CGRP signaling.
EC Johnson EC et al. (2005). J Exp Biol. 208, 1239-1246.

TGF-beta-induced apoptosis in endothelial cells mediated by M6P/IGFII-R and mini-plasminogen.
Leksa V et al. (2005) J Cell Sci. 118, 4577-86.

Altered intracellular sorting signals do not influence the efficacy of genetic melanoma vaccines incorporating helper determinants in mice.
Bruck J et al. (2005). J Gene Med. 7, 613-20,

Drosophila CAP-D2 is required for condensin complex stability and resolution of sister chromatids.
Savvidou E et al. (2005) J Cell Sci. 118, 2529-43.

A tripeptide mimetic of von Willebrand factor residues 981-983 enhances platelet adhesion to fibrinogen by signaling through integrin alpha(IIb)beta3.
Litjens PE et al. (2005) J Thromb Haemost. 3, 1274-83.

Microsatellites and RAPD Markers to Study Genetic Relationships Among Cowpea Breeding Lines.
D Diouf et al. (2005) Genetic Res Crop Evol 52, 1057–1067.

Cyclic nucleotide phosphodiesterases in Drosophila melanogaster.
Day JP et al. (2005) Biochem J. 388, 333-42.

Peripheral CD8+ T cell tolerance against melanocytic self-antigens in the skin is regulated in two steps by CD4+ T cells and local inflammation: implications for the pathophysiology of vitiligo.
J Steitz et al. (2005) J Invest Dermatol. 124, 144-50.

Transient receptor potential-like channels are essential for calcium signaling and fluid transport in a Drosophila epithelium.
MacPherson MR et al. (2005) Genetics 169, 1541-52.

Chitinase and Fizz Family Members Are a Generalized Feature of Nematode Infection with Selective Upregulation of Ym1 and Fizz1 by Antigen-Presenting Cells.
Nair MG et al. (2005) Infect Immun. 73, 385-394.

A novel, mouse mammary tumor virus encoded protein with Rev-like properties.
Indik S et al. (2005) Virol. 337, 1-6.

Altered intracellular sorting signals do not influence the efficacy of genetic melanoma vaccines incorporating helper determinants in mice.
Bruck J et al. (2005). J Gene Med. 7, 613-20.

Identification of the VirB4-VirB8-VirB5-VirB2 pilus assembly sequence of type IV secretion systems.
Yuan Q et al. (2005). J Biol Chem. 280, 26349-59.

Copper binding to prion octarepeat peptides, a combined metal chelate affinity and immunochemical approaches.
Todorova-Balvay D et al. (2005). Journal of Chromatography B 818, 75-82.

De-glycosylation of Pichia pastoris-produced Schistosoma mansoni cathepsin B eliminates non-specific reactivity with IgG in normal human serum.
Stack CM et al. (2005). J Immunol Methods. 304, 151-7.

Expression, purification, and characterization of recombinant cyanovirin-N for vaginal anti-HIV microbicide development.
Colleluori DM et al. (2005). Protein Expr Purif. 39, 229-36.

The binding site in beta2-glycoprotein I for ApoER2’ on platelets is located in domain V.
van Lummel M et al. (2005). J Biol Chem 280, 36729-36.


Navigation