Selected published research using custom-made peptides, antibodies, synthetic genes, or bioanalysis from Genosphere Biotechnologies.

 

 

 

Unraveling flp-11/flp-32 dichotomy in nematodes.

Atkinson, L.E., Miskelly, I.R., Moffett, C.L., McCoy, C.J., Maule, A.G., Marks, N.J. and Mousley, A., 2016.

International Journal for Parasitology, 46(11), pp.723-736.

doi:  10.1016/j.ijpara.2016.05.010

FLP-11; FLP-32; FMRFamide-like peptide; Nematode; Neuropeptide; Neuropeptide signalling; flp-11; flp-32

Abstract

FMRFamide-like peptide (FLP) signalling systems are core to nematode neuromuscular function. Novel drug discovery efforts associated with nematode FLP/FLP receptor biology are advanced through the accumulation of basic biological data that can reveal subtle complexities within the neuropeptidergic system. This study reports the characterisation of FMRFamide-like peptide encoding gene-11 (flp-11) and FMRFamide-like peptide encoding gene-32 (flp-32), two distinct flp genes which encode the analogous peptide, AMRN(A/S)LVRFamide, in multiple nematode species - the only known example of this phenomenon within the FLPergic system of nematodes. Using bioinformatics, in situ hybridisation, immunocytochemistry and behavioural assays we show that: (i) flp-11 and -32 are distinct flp genes expressed individually or in tandem across multiple nematode species, where they encode a highly similar peptide; (ii) flp-11 does not appear to be the most widely expressed flp in Caenorhabditis elegans; (iii) in species expressing both flp-11 and flp-32, flp-11 displays a conserved, restricted expression pattern across nematode clades and lifestyles; (iv) in species expressing both flp-11 and flp-32, flp-32 expression is more widespread and less conserved than flp-11; (v) in species expressing only flp-11, the flp-11 expression profile is more similar to the flp-32 profile observed in species expressing both; and (vi) FLP-11 peptides inhibit motor function in multiple nematode species. The biological significance and evolutionary origin of flp-11 and -32 peptide duplication remains unclear despite attempts to identify a common ancestor; this may become clearer as the availability of genomic data improves. This work provides insight into the complexity of the neuropeptidergic system in nematodes, and begins to examine how nematodes may compensate for structural neuronal simplicity. From a parasite control standpoint, this work underscores the importance of basic biological data, and has wider implications for the utility of C. elegans as a model for parasite neurobiology.

TRAIP is a PCNA-binding ubiquitin ligase that protects genome stability after replication stress

Hoffmann, S., Smedegaard, S., Nakamura, K., Mortuza, G.B., Räschle, M., Ibañez, D.O.A., Oka, Y., Feng, Y., Blanco, F.J., Mann, M. and Montoya, G., 2016.

The Journal of cell biology, 212(1), 63.

doi:  10.1083/jcb.201506071

Proliferating Cell Nuclear Antigen ; TRAIP protein, human ; Ubiquitin-Protein Ligases

Abstract

Cellular genomes are highly vulnerable to perturbations to chromosomal DNA replication. Proliferating cell nuclear antigen (PCNA), the processivity factor for DNA replication, plays a central role as a platform for recruitment of genome surveillance and DNA repair factors to replication forks, allowing cells to mitigate the threats to genome stability posed by replication stress. We identify the E3 ubiquitin ligase TRAIP as a new factor at active and stressed replication forks that directly interacts with PCNA via a conserved PCNA-interacting peptide (PIP) box motif. We show that TRAIP promotes ATR-dependent checkpoint signaling in human cells by facilitating the generation of RPA-bound single-stranded DNA regions upon replication stress in a manner that critically requires its E3 ligase activity and is potentiated by the PIP box. Consequently, loss of TRAIP function leads to enhanced chromosomal instability and decreased cell survival after replication stress. These findings establish TRAIP as a PCNA-binding ubiquitin ligase with an important role in protecting genome integrity after obstacles to DNA replication.

 

The corticotropin-releasing factor-like diuretic hormone 44 (DH 44) and kinin neuropeptides modulate desiccation and starvation tolerance in Drosophila melanogaster.

Cannell, E., Dornan, A.J., Halberg, K.A., Terhzaz, S., Dow, J.A. and Davies, S.A., 2016.

Peptides 80, pp.96-107.

doi:  10.1016/j.peptides.2016.02.004

DH(44); Desiccation; Drosophila melanogaster; Kinin; Neuropeptide receptor; Starvation

Abstract

Malpighian tubules are critical organs for epithelial fluid transport and stress tolerance in insects, and are under neuroendocrine control by multiple neuropeptides secreted by identified neurons. Here, we demonstrate roles for CRF-like diuretic hormone 44 (DH44) and Drosophila melanogaster kinin (Drome-kinin, DK) in desiccation and starvation tolerance. Gene expression and labelled DH44 ligand binding data, as well as highly selective knockdowns and/or neuronal ablations of DH44 in neurons of the pars intercerebralis and DH44 receptor (DH44-R2) in Malpighian tubule principal cells, indicate that suppression of DH44 signalling improves desiccation tolerance of the intact fly. Drome-kinin receptor, encoded by the leucokinin receptor gene, LKR, is expressed in DH44 neurons as well as in stellate cells of the Malpighian tubules. LKR knockdown in DH44-expressing neurons reduces Malpighian tubule-specific LKR, suggesting interactions between DH44 and LK signalling pathways. Finally, although a role for DK in desiccation tolerance was not defined, we demonstrate a novel role for Malpighian tubule cell-specific LKR in starvation tolerance. Starvation increases gene expression of epithelial LKR. Also, Malpighian tubule stellate cell-specific knockdown of LKR significantly reduced starvation tolerance, demonstrating a role for neuropeptide signalling during starvation stress.

A cardiac mitochondrial cAMP signaling pathway regulates calcium accumulation, permeability transition and cell death

Z Wang, D Liu, A Varin, V Nicolas, D Courilleau, P Mateo, C Caubere, P Rouet, A-M Gomez, G Vandecasteele, R Fischmeister, and C Brenner,  2016

Cell death & disease 7(4), p.e2198.

doi:  10.1038/cddis.2016.106

Abstract

Although cardiac cytosolic cyclic 3′,5′-adenosine monophosphate (cAMP) regulates multiple processes, such as beating, contractility, metabolism and apoptosis, little is known yet on the role of this second messenger within cardiac mitochondria. Using cellular and subcellular approaches, we demonstrate here the local expression of several actors of cAMP signaling within cardiac mitochondria, namely a truncated form of soluble AC (sACt) and the exchange protein directly activated by cAMP 1 (Epac1), and show a protective role for sACt against cell death, apoptosis as well as necrosis in primary cardiomyocytes. Upon stimulation with bicarbonate (HCO3) and Ca2+, sACt produces cAMP, which in turn stimulates oxygen consumption, increases the mitochondrial membrane potential (ΔΨm) and ATP production. cAMP is rate limiting for matrix Ca2+ entry via Epac1 and the mitochondrial calcium uniporter and, as a consequence, prevents mitochondrial permeability transition (MPT). The mitochondrial cAMP effects involve neither protein kinase A, Epac2 nor the mitochondrial Na+/Ca2+ exchanger. In addition, in mitochondria isolated from failing rat hearts, stimulation of the mitochondrial cAMP pathway by HCO3 rescued the sensitization of mitochondria to Ca2+-induced MPT. Thus, our study identifies a link between mitochondrial cAMP, mitochondrial metabolism and cell death in the heart, which is independent of cytosolic cAMP signaling. Our results might have implications for therapeutic prevention of cell death in cardiac pathologies.

Identification of Phosphorylation Sites Regulating sst3 Somatostatin Receptor Trafficking.

Lehmann, A., Kliewer, A., Günther, T., Nagel, F. and Schulz, S., 2016

Molecular Endocrinology, 30(6), 645-659.

doi:  10.1210/me.2015-1244

Abstract

The human somatostatin receptor 3 (sst3) is expressed in about 50% of all neuroendocrine tumors and hence a promising target for multireceptor somatostatin analogs. The sst3 receptor is unique among ssts in that it exhibits a very long intracellular C-terminal tail containing a huge number of potential phosphate acceptor sites. Consequently, our knowledge about the functional role of the C-terminal tail in sst3 receptor regulation is very limited. Here, we have generated a series of phosphorylation-deficient mutants that enabled us to determine crucial sites for its agonist-induced β-arrestin mobilization, internalization, and down-regulation. Based on this information, we generated phosphosite-specific antibodies for C-terminal Ser(337)/Thr(341), Thr(348), and Ser(361) that enabled us to investigate the temporal patterns of sst3 phosphorylation and dephosphorylation. We found that the endogenous ligand somatostatin induced a rapid and robust phosphorylation that was completely blocked by the sst3 antagonist NVP-ACQ090. The stable somatostatin analogs pasireotide and octreotide promoted clearly less phosphorylation compared with somatostatin. We also show that sst3 phosphorylation occurred within seconds to minutes, whereas dephosphorylation of the sst3 receptor occurred at a considerable slower rate. In addition, we also identified G protein-coupled receptor kinases 2 and 3 and protein phosphatase 1α and 1β as key regulators of sst3 phosphorylation and dephosphorylation, respectively. Thus, we here define the C-terminal phosphorylation motif of the human sst3 receptor that regulates its agonist-promoted phosphorylation, β-arrestin recruitment, and internalization of this clinically relevant receptor.


Nuclear fallout provides a new link between aPKC and polarized cell trafficking.

Calero-Cuenca, F.J. et al. (2016) BMC biology 14(1), p.32

doi  l  Europe PMC

Keywords:  aPKC; Cell polarity; Cell trafficking; Nuclear fallout

 

Immuno-affinity purification of PglPGIP1, a polygalacturonase-inhibitor protein from pearl millet: studies on its inhibition of fungal polygalacturonases and role in resistance against the downy mildew pathogen.

Prabhu, S. A. et al. (2015) Molecular biology reports 42(6), pp.1123-1138.

doi  l  Europe PMC

Keywords:  Chemical deglycosylation; Confocal immunolocalization; Peptide mass fingerprinting; Plant–pathogen interaction; Quantitative real-time PCR; Two-dimensional gel electrophoresis

 

The structure of the TBCE/TBCB chaperones and α-tubulin complex shows a tubulin dimer dissociation mechanism. 

Serna M et al. (2015)  Journal of Cell Science 128(9), pp. 1824-1834

doi  l  Europe PMC

Keywords: Tubulin; Protein degradation; Chaperones; Folding cofactors; Microtubule

 

EMMPRIN regulates β1 integrin-mediated adhesion through Kindlin-3 in human melanoma cells.

Delyon J et al. (2015) Experimental dermatology, 24(6),  pp. 443-448

doi  l  Europe PMC

Keywords:  EMMPRIN; Kindlin-3; cell adhesion; melanoma; β1 integrin

 

TopBP1 interacts with BLM to maintain genome stability but is dispensable for preventing BLM degradation.

Blackford A N et al. (2015) Mol cell 57, 1133-1141

doi Europe PMC

 

Identification of a Taraxacum brevicorniculatum rubber elongation factor protein that is localized on rubber particles and promotes rubber biosynthesis.
Laibach N et al. (2015) The Plant J 82, 609-620.

doi Europe PMC

 

Use of autoantigen-loaded phosphatidylserine-liposomes to arrest autoimmunity in type 1 diabetes.
Pujol-Autonell I et al. (2015) PloS ONE 10.6, e0127057

 doi Europe PMC

 

Influence of Ligands in Metal Nanoparticle Electrophoresis for the Fabrication of Biofunctional Coatings.
Streich C et al. (2015) Appl Surface Sci 348, 92–99

doi Europe PMC

 

Carbon nanotubes and graphene modified screen-printed carbon electrodes as sensitive sensors for the determination of phytochelatins in plants using liquid chromatography with amperometric detection.
Dago A et al. (2015) J Chromatography A 1409, 210–217

doi Europe PMC

 

Klf15 Is Critical for the Development and Differentiation of Drosophila Nephrocytes.
Ivy JR et al. (2015) PLoS ONE 10.8, e0134620

doi Europe PMC

 

New insight into the structure and function of Hfq C-terminus.
Fortas E et al. (2015) Bioscience Rep 35 (2).

Memory and PTPIP51 - A new protein in hippocampus and cerebellum.
Brobeil A et al. (2015) Mol Cell Neurosci 64, 61-73.

Signal Transduction by a Fungal NOD-Like Receptor Based on Propagation of a Prion Amyloid Fold.
Daskalov A et al. (2015) PLoS Biol 13, e1002059

The adhesive properties of the Staphylococcus lugdunensis multifunctional autolysin AtlL and its role in biofilm formation and internalization.
Hussain M et al. (2015) Int J Med Microbiol 305, 129-39

β-Casein(94-123)-derived peptides differently modulate production of mucins in intestinal goblet cells.
Plaisancié P et al. (2015) J Dairy Res 82, 36-46

Full membrane spanning self-assembled monolayers as model systems for UHV-based studies of cell-penetrating peptides.
Franz J et al. (2015) Biointerphases 10(1) , 019009.

Src family kinases interfere with dimerization of STAT5A through a phosphotyrosine-SH2 domain interaction.
Fahrenkamp D et al. (2015) Cell Communication and Signaling 13, 10

Chloride channels in stellate cells are essential for uniquely high secretion rates in neuropeptide-stimulated Drosophila diuresis.
Cabrero P et al. (2014) Proc Natl Acad Sci U S A 111,14301-14306.

Escherichia coli Common Pilus (ECP) Targets Arabinosyl Residues in Plant Cell Walls to Mediate Adhesion to Fresh Produce Plants.
Rossez Y et al. (2014) J Biol Chem 289, 34349.

The role of metalloproteinase ADAM17 in regulating ICOS ligand-mediated humoral immune responses.
Marczynska J et al. (2014) J Immunol 193, 2753-63

Identification of a mitochondrial external NADPH dehydrogenase by overexpression in transgenic Nicotiana sylvestris.
Michalecka AM et al. (2014) Plant J 37, 415-25.

CD31 is a key coinhibitory receptor in the development of immunogenic dendritic cells.
Clement M et al. (2014) Proc Natl Acad Sci U S A 111, E1101-10.

An ancient oxidoreductase making differential use of its cofactors.
D Blüher et al. (2014) Biol Chem 395, 855–869

[The acidic C-terminal tail of the GyrA subunit moderates the DNA supercoiling activity of Bacillus subtilis gyrase.
Lanz MA et al. (2014) J Biol Chem 289, 12275-85

A polymerase chain reaction and enzyme linked immunosorbent assay based approach for diagnosis and differentiation between vaccinated and infected cattle with Mycobacterium bovis.
Sabry M et al. (2014) J Pharm Bioallied Sci 6, 115-21

Study on Aβ34 biology and detection in transgenic mice brains.
Caillava C et al. (2014) Neurobiol Aging 35, 1570-81

Novel cleavable cell-penetrating peptide-drug conjugates: synthesis and characterization.
Lelle M et al. (2014) J Pept Sci 20, 323-33

Effects of synthetic analogues of human opiorphin on rat brain opioid receptors.
Benyhe Z et al. (2014) J Physiol Pharmacol 65, 525-30

Arabidopsis Thaliana AXR4 Recombinant Protein Expression for Polyclonal Antibody Production
Norliza AB et al. (2014) Asian J of Plant Biol 1 , 34-38

Optimising and Evaluating the Characteristics of a Multiple Antigen ELISA for Detection of Mycobacterium bovis Infection in a Badger Vaccine Field Trial.
Aznar I et al. (2014) PloS one 9, e100139.

A novel tumor suppressor function of Kindlin-3 in solid cancer.
Djaafri I et al. (2014) Oncotarget 5, 8970-85.

Suppression of NDA-type alternative mitochondrial NAD(P)H dehydrogenases in arabidopsis thaliana modifies growth and metabolism, but not high light stimulation of mitochondrial electron transport.
Wallström SV et al. (2014) Plant Cell Physiol 55, 881-96

Polylactide block Polypeptide block Polylactide Copolymer Nanoparticles with Tunable Cleavage and Controlled Drug Release.
Dorresteijn R et al. (2014) Adv Funct Mater 24, 4026–4033

Mice deficient for the epidermal dermokine β and γ isoforms display transient cornification defects.
Leclerc EA et al. (2014) J Cell Sci 127, 2862-72

The Expression of the Hepatocyte SLAMF3 (CD229) Receptor Enhances the Hepatitis C Virus Infection.
Cartier F et al. (2014) PLoS ONE 9, e99601

Regulation of the chloroplastic copper chaperone (CCS) and cuprozinc superoxide dismutase (CSD2) by alternative splicing and copper excess in Glycine max.
Sagasti S et al. (2014) Funct Plant Biol 41, 144–155

Dose-dependent inhibition of BACE-1 by the monoterpenoid 2,3,4,4-tetramethyl-5-methylenecyclopent-2-enone in cellular and mouse models of Alzheimer’s disease.
Videira R et al. (2014) J Nat Prod 77, 1275-9

Development of Peptide-Based Lineage-Specific Serology for Chronic Chagas Disease: Geographical and Clinical Distribution of Epitope Recognition.
Bhattacharyya T et al. (2014) PLoS Negl Trop Dis 8, e2892

Interferon gamma peptidomimetic targeted to hepatic stellate cells ameliorates acute and chronic liver fibrosis in vivo
Bansal R et al. (2014) J Controlled Release 179, 18-24

Molecular rules for chemo-and regio-selectivity of Candida antarctica lipase B in peptide acylation reactions
Ferrari F et al. (2014) J Mol Catalysis B: Enzymatic 101, 122-132

Formation Constants of Copper(II) Complexes with Tripeptides Containing Glu, Gly, and His: Potentiometric Measurements and Modeling by Generalized Multiplicative Analysis of Variance.
Khoury RR et al. (2014) Inorg Chem 53, 1278-87

Absence of cell surface expression of human ACE leads to perinatal death.
Michaud A et al. (2014) Hum Mol Genet 23, 1479-91

A high-performance liquid chromatography assay for Dyrk1a, a Down syndrome-associated kinase.
Bui LC et al. (2014) Anal Biochem 449172-8

Surface plasmon resonance analysis of the binding mechanism of pharmacological and peptidic inhibitors to human somatic angiotensin I-converting enzyme.
Zidane F et al. (2013) Biochemistry 52, 8722-31

Signal Peptide Cleavage from GP5 of PRRSV: A Minor Fraction of Molecules Retains the Decoy Epitope, a Presumed Molecular Cause for Viral Persistence.
Thaa B et al. (2013) PLoS One. 8, e65548.

The Tll0287 protein is a hemoprotein associated with the PsbA2-Photosystem II complex in Thermosynechococcus elongatus.
Boussac A et al. (2013) Biochimica et Biophysica Acta 1827, 1174-82

Amino acid transporters expression in acinar cells is changed during acute pancreatitis
Rooman I et al. (2013) Pancreatology 13, 475–485

Calcium-sensing receptor activation increases cell-cell adhesion and β-cell function. Hills CE et al. (2013) Cell Physiol Biochem 30, 575-586.

High-resolution spatio-temporal bioactivity of a novel peptide revealed by optical imaging in rat orbitofrontal cortex in vitro: Possible implications for neurodegenerative diseases.
Badin AS et al. (2013) Neuropharmacology 73, 10-18

Hydrolysis of milk-derived bioactive peptides by cell-associated extracellular peptidases of Streptococcus thermophilus.
Hafeez Z et al. (2013) Appl Microbiol Biotechnol 97, 9787-99

Promoter-dependent expression of the fungal transporter HcPT1.1 under Pi shortage and its spatial localization in ectomycorrhiza.
Garcia K et al. (2013) Fungal Genet Biol 58, 53-61

A necrodane monoterpenoid from Lavandula luisieri essential oil as a cell-permeable inhibitor of BACE-1, the β-secretase in Alzheimer’s disease.
Videira, R et al. (2013) Flavour Fragr. J 28, 380–388

Cathepsin D activity and selectivity in the acidic conditions of a tumor microenvironment: Utilization in the development of a novel Cathepsin D substrate for simultaneous cancer diagnosis and therapy
Achour O et al. (2013) Biochimie 95, 2010–2017

Regulation of the chloroplastic copper chaperone (CCS) and cuprozinc superoxide dismutase (CSD2) by alternative splicing and copper excess in Glycine max
Sagasti S et al. (2013) Functional Plant Biology 41, 144-155

Can bismuth film screen printed carbon electrodes be used to study complexation?
Sosa V et al. (2013) Talanta 107, 356-60

Perturbation of invadolysin disrupts cell migration in zebrafish (Danio rerio)
Vass S et al. (2013) Exp Cell Res 319, 1198–1212.

Glutaminase isoenzymes as key regulators in metabolic and oxidative stress against cancer.
Matés JM et al. (2013) Curr Mol Med 13, 514-34

Opiorphin Secretion Pattern in Healthy Volunteers: Gender Difference and Organ Specificity.
Dufour E et al. (2013) Biochem Anal Biochem 2, 136

Additive Toxicity of β-Amyloid by a Novel Bioactive Peptide In Vitro: Possible Implications for Alzheimer’s Disease.
Garcia-Rates S et al. (2013) PLoS One 8, e54864.

Ataxia with cerebellar lesions in mice expressing chimeric PrP-Dpl protein.
Lemaire-Vieille C et al. (2013) J Neurosci. 33, 1391-1399.

Detection and quantification of staphylococcal enterotoxin A in foods with specific and sensitive polyclonal antibodies
Clarisse T et al. (2013) Food Control 32, 255–261

Measurement and modeling of acid dissociation constants of tri-peptides containing Glu, Gly, and His using potentiometry and generalized multiplicative analysis of variance
Khoury RR et al. (2013) Dalton Trans. 42, 2940-2947

A novel bioactive peptide from yoghurts modulates expression of the gel-forming MUC2 mucin as well as population of goblet cells and Paneth cells along the small intestine.
Plaisancié P et al. (2013) J Nutr Biochem. 24, 213-21

flp-32 Ligand/Receptor Silencing Phenocopy Faster Plant Pathogenic Nematodes.
Atkinson LE et al. (2013) PLoS Pathog 9, e1003169

GSK3-mediated VDAC phosphorylation controls outer mitochondrial membrane permeability during lipid accumulation.
Martel C et al. (2013) Hepatology 57, 93-102.

Expression and localization of glutamate-gated chloride channel variants in honeybee brain (Apis mellifera).
Demares F et al. (2013) Insect Biochem Mol Biol. 43, 115-24

The metalloprotease meprin β generates amino terminal truncated Aβ-peptide species.
Bien J et al. (2012) J Biol Chem. 287, 33304-13.

Competitive binding of UBPY and ubiquitin to the STAM2 SH3 domain revealed by NMR.
Lange A et al. (2012) FEBS Lett. 586, 3379-84

Staphylococcus aureus Panton-Valentine leukocidin induces an inflammatory response in human phagocytes via the NLRP3 inflammasome.
Holzinger D et al. (2012) J Leukoc Biol. 92, 1069-81.

ANT-VDAC1 interaction is direct and depends on ANT isoform conformation in vitro.
Allouche M et al. (2012) Biochem Biophys Res Commun. 429, 12-17

Dissecting mannose 6-phosphate-insulin-like growth factor 2 receptor complexes that control activation and uptake of plasminogen in cells.
Leksa V et al. (2012) J Biol Chem. 287, 22450-62

PTPIP51 in protein interactions: regulation and in situ interacting partners.
Brobeil A et al. (2012) Cell Biochem Biophys. 63, 211-22.

NMR structure of hsp12, a protein induced by and required for dietary restriction-induced lifespan extension in yeast.
Herbert AP et al. (2012) PLoS One. 7, e41975

Investigations of the supramolecular structure of individual diphenylalanine nano- and microtubes by polarized Raman microspectroscopy.
Lekprasert B et al. (2012) Biomacromolecules. 13, 2181-7

Mammalian glutaminase Gls2 gene encodes two functional alternative transcripts by a surrogate promoter usage mechanism.
Martín-Rufián M et al. (2012) PLoS One. 7, e38380

Identification, localization and function of glutamate-gated chloride channel receptors in the honeybee brain.
El Hassani AK et al. (2012) Eur J Neurosci. 36, 2409-20

Opiorphin highly improves the specific binding and affinity of MERF and MEGY to rat brain opioid receptors.
Tóth F et al. (2012) Regul Pept. 178, 71-5

A CD31-derived peptide prevents angiotensin II-induced atherosclerosis progression and aneurysm formation.
Fornasa G et al. (2012) Cardiovasc Res. 94, 30-7

Targeted and intracellular triggered delivery of therapeutics to cancer cells and the tumor microenvironment: impact on the treatment of breast cancer.
Moura V et al. (2012) Breast Cancer Res Treat. 133, 61-73

Chlamydia co-opts the rod shape-determining proteins MreB and Pbp2 for cell division.
Ouellette SP et al. (2012) Mol Microbiol. 85, 164-78

Marginal zone B cells regulate antigen-specific T cell responses during infection.
Bankoti R et al. (2012) J Immunol. 188, 3961-71

Functional and conformational changes in the aspartic protease cardosin A induced by TFE.
Fraga AS et al. (2012) Int J Biol Macromol. 50, 323-30

Increased expression of VDAC1 sensitizes carcinoma cells to apoptosis induced by DNA cross-linking agents.
Sharaf El Dein O et al. (2012) Biochem Pharmacol. 83, 1172-82

Mechanism and function of Drosophila capa GPCR: a desiccation stress-responsive receptor with functional homology to human neuromedinU receptor.
Terhzaz S et al. (2012) PLoS One. 7, e29897

Calcium-induced folding of intrinsically disordered repeat-in-toxin (RTX) motifs via changes of protein charges and oligomerization states.
Sotomayor-Pérez AC et al. (2011) J Biol Chem. 286, 16997-7004

Loss-of-function point mutations associated with renal tubular dysgenesis provide insights about renin function and cellular trafficking.
Michaud A et al. (2011) Hum Mol Genet. 20, 301-11

Expression of Drosophila Cabut during early embryogenesis, dorsal closure and nervous system development.
Belacortu Y et al. (2011) Gene Expr Patterns. 11, 190-201.

Use of a multiplex enzyme-linked immunosorbent assay to detect a subpopulation of Mycobacterium bovis-infected animals deemed negative or inconclusive by the single intradermal comparative tuberculin skin test.
Whelan C et al. (2011) J Vet Diagn Invest. 23, 499-503.

Peptide-regulated gene depletion system developed for use in Streptococcus pneumoniae.
Berg KH et al. (2011) J Bacteriol. 193, 5207-15.

Distinct expression, localization and function of two Rab7 proteins encoded by paralogous genes in a free-living model eukaryote.
Osińska M et al. (2011) Acta Biochim Pol. 58, 597-607.

Plk5, a Polo-box domain-only protein with specific roles in neuron differentiation and glioblastoma suppression.
de Cárcer G et al. (2011) Mol Cell Biol. 31, 1225-39.

Expression and Localization of Aquaporin 1a in the Sea-Bass (Dicentrarchus labrax) during Ontogeny.
Giffard-Mena I et al. (2011) Front Physiol. 2, 34

Characterization of the recombinant copper chaperone (CCS) from the plant Glycine (G.) max.
Sagasti S et al. (2011) Metallomics 3, 169-75.

Schistosome I/Lamides—a new family of bioactive helminth neuropeptides.
McVeigh P et al. (2011) Int J Parasitol. 8, 905-13.

Shewasin A, an active pepsin homolog from the bacterium Shewanella amazonensis.
Simões et al. (2011) FEBS J. 278, 3177-86.

Sequence variability of the pattern recognition receptor Mermaid mediates specificity of marine nematode symbioses.
Bulgheresi S et al. (2011) ISME J. 5, 986-98.

Soluble M6P/IGF2R Released by TACE Controls Angiogenesis via Blocking Plasminogen Activation.
Leksa V et al. (2011) Circ Res. 108, 676-85.

Deletion of UCP2 in iNOS deficient mice reduces the severity of the disease during experimental autoimmune encephalomyelitis.
Aheng C et al. (2011) PLoS ONE 6, e22841.

Peptides derived from the bifunctional kinase/RNase enzyme IRE1alpha modulate IRE1alpha activity and protect cells from endoplasmic reticulum stress.
Bouchecareilh M et al. (2011) FASEB J. 25, 3115-29.

Plasmacytoid dendritic cells play a major role in apoptotic leukocyte-induced immune modulation.
Bonnefoy F et al. (2011) J Immunol. 186, 5696-705.

Secretory leukocyte protease inhibitor (SLPI) is, like its homologue trappin-2 (Pre-Elafin), a transglutaminase substrate.
Baranger K et al. (2011) PLoS ONE 6, e20976.

Transcriptome and metabolite profiling show that APETALA2a is a major regulator of tomato fruit ripening.
Karlova R et al. (2011) Plant Cell. 23, 923-41.

Inhibition of Nuclear Translocation of Calcineurin Suppresses T-Cell Activation and Prevents Acute Rejection of Donor Hearts.
Panther F et al. (2011) Transplantation 91, 597-604.

Use of allostery to identify inhibitors of calmodulin-induced activation of Bacillus anthracis edema factor.
Laine E et al. (2010) Proc Natl Acad Sci U S A. 107, 11277-82.

Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y˙) involved in ribonucleotide reduction.
Stolle P et al. (2010) FEBS J. 277, 4849-62

Serine protease-mediated host invasion by the parasitic nematode Steinernema carpocapsae.
Toubarro D et al. (2010) J Biol Chem. 285, 30666-75.

A combined zinc/cadmium sensor and zinc/cadmium export regulator in a heavy metal pump.
Baekgaard L et al. (2010) J Biol Chem. 285, 31243-52

Calcium-induced folding and stabilization of the intrinsically disordered RTX domain of the CyaA toxin.
Chenal A et al. (2010) Biophys J. 99, 3744-53

The IkappaB kinase inhibitor nuclear factor-kappaB essential modulator-binding domain peptide for inhibition of injury-induced neointimal formation.
Grassia G et al. (2010) Arterioscler Thromb Vasc Biol. 30, 2458-66

Nondestructive Raman and atomic force microscopy measurement of molecular structure for individual diphenylalanine nanotubes.
Lekprasert B et al. (2010) Opt Lett. 35, 4193-5.

Mislocalization of mitochondria and compromised renal function and oxidative stress resistance in Drosophila SesB mutants.
Terhzaz S et al. (2010) Physiol Genomics 41, 33-41.

Systemically active human opiorphin is a potent yet non-addictive analgesic without drug tolerance effects.
Rougeot C et al. (2010) J Physiol Pharmacol. 61, 483-90.

Sensitive liquid chromatography/tandem mass spectrometry assay for absolute quantification of ITIH4-derived putative biomarker peptides in clinical serum samples.
van den Broek I et al. (2010) Rapid Commun Mass Spectrom. 24, 1842-50.

Salt stress causes peroxisome proliferation, but inducing peroxisome proliferation does not improve NaCl tolerance in Arabidopsis thaliana.
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Microsatellites and RAPD Markers to Study Genetic Relationships Among Cowpea Breeding Lines.
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Identification of the VirB4-VirB8-VirB5-VirB2 pilus assembly sequence of type IV secretion systems.
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Expression, purification, and characterization of recombinant cyanovirin-N for vaginal anti-HIV microbicide development.
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