In many instances, raising antibodies in chicken has proven essential. Where genetically distant species is required e.g. conserved mammalian proteins, Genosphere Biotechnologies will produce your antibodies in chicken. IgY antibodies may be isolated from circulating blood of chicken or extracted and purified in high yields from egg yolks of immunized laying hens.
Pre-immune eggs are collected and control IgYs are purified. Following four immunizations over a 70-days period, 2-4 eggs are collected, yolks are pooled and we isolate the IgY fraction (purity 90-95%). The final antibodies preparation is then titered by ELISA.
Protocols may be extended beyond the initial dispatching and more eggs would be collected.
In addition, the IgY fraction may be further purified by affinity chromatography using a peptide or antigen-column.
For large scale IgY production, multiple hen immunization, and timely supply over long periods, please contact us with your project details and we will design the most efficient strategy for you.