Phospho-Specific Antibodies

Fig 7. ELISA response of the phospho-specific antibodies against (a) the phosphorylated sequence and (b) the non-phosphorylated sequence.
Click on figure to enlarge

This service allows the production of antibodies that will specifically recognize the phosphorylated state of your target protein. The approach includes the synthesis and purification of phosphorylated and non-phosphorylated peptides, immunisation, fabrication of two affinity columns for successive purification. Extensive ELISA on phospho-specific antibodies as well as on the fraction specific to the non-phosphorylated state is performed. We have successfully utilized this method with other post-translational modifications including acetylation and methylation.