Custom Services
For Research
Custom Antibody Development
Monoclonal antibodies
Monoclonal antibodies
| Designation | Host | Antigen | Service | Deliverable | Lead time |
|---|---|---|---|---|---|
|
Anti-protein monoclonal antibody (you supply your protein antigen) |
Mouse | Customer provides: • your purified protein • >90% purity • >10 KDa • Minimum amount: 5 mg |
• Immunisation and sera collection • Titer evaluation (ELISA) • Fusion and hybridoma development • ELISA screening and cloning • Isotype determination • Ascites production and purification (2 clones) |
• 2 mg purified mAb • ELISA result - Titer guaranteed >1:50:000 • Isotype determination • Project report |
23-26 weeks |
| Anti-protein monoclonal antibody (we express your protein antigen) |
Mouse |
Custom Protein Expression: |
• Protein antigen expression and purification • Immunisation and sera collection • Titer evaluation (ELISA) • Fusion and hybridoma development • ELISA screening and cloning • Isotype determination • Ascites production and purification (2 clones) |
• 2 mg purified mAb • ELISA result - Titer guaranteed >1:50:000 • Isotype determination • Project report • 0.5 to 1 mg of purified protein |
25-28 weeks |
|
Anti-peptide monoclonal antibody (we synthesise the peptide)
|
Mouse | Custom Peptide synthesis: We prepare synthetic peptide 12-20 aa, with terminal cys for conjugation Immunogen: KLH-peptide conjugate |
• Target protein analysis and peptide design • Synthesis, purification & conjugation of your peptide (purity >90%) • Immunisation and sera collection • Titer evaluation (ELISA) • Fusion and hybridoma development • ELISA screening and cloning • Isotype determination • Ascites production and purification (2 clones) |
• 2 mg purified mAb • ELISA result - Titer guaranteed >1:50:000 • Isotype determination • Project report • 2 mg free peptide, purity >90% LC & MS analysis |
24-28 weeks |