Anticorps monoclonaux

 

Designation Host Antigen Service Deliverable Lead time

Anti-protein monoclonal antibody

(you supply your protein antigen)

Mouse Customer provides:
your purified protein
>90% purity
>10 KDa
Minimum amount:   5 mg
Immunisation and sera collection
Titer evaluation (ELISA)
Fusion and hybridoma development
ELISA screening and cloning
Isotype determination
Ascites production and purification (2 clones)
• 2 mg purified mAb
ELISA result - Titer guaranteed >1:50:000
Isotype determination
Project report
23-26 weeks

Anti-protein monoclonal antibody

(we express your protein antigen)

Mouse

Custom Protein Expression:
DNA sequence design for optimum protein expression, gene synthesis,
vector construction, protein expression and purification

Protein antigen expression and purification
Immunisation and sera collection
Titer evaluation (ELISA)
Fusion and hybridoma development
ELISA screening and cloning
Isotype determination
Ascites production and purification (2 clones)
• 2 mg purified mAb
ELISA result - Titer guaranteed >1:50:000
Isotype determination
Project report
0.5 to 1 mg of purified protein
25-28 weeks

Anti-peptide monoclonal antibody

(we synthesise the peptide)

 

Mouse Custom Peptide synthesis:
We prepare synthetic peptide
12-20 aa, with terminal cys for conjugation
Immunogen: KLH-peptide conjugate
Target protein analysis and peptide design
Synthesis, purification & conjugation of your peptide (purity >90%)
Immunisation and sera collection
Titer evaluation (ELISA)
Fusion and hybridoma development
ELISA screening and cloning
Isotype determination
Ascites production and purification (2 clones)
• 2 mg purified mAb
ELISA result - Titer guaranteed >1:50:000
Isotype determination
Project report
2 mg free peptide, purity >90% LC & MS analysis
24-28 weeks

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