This service includes the following features:
Antigens may be supplied in solution, lyophilized, as gel slice, inclusion bodies, insoluble aggregates, GST/His fusions, protein conjugates, etc. It should be noted that small proteins (<10 kDa), peptides, organic molecules need to be conjugated to a carrier protein in order to elicit a good immune response in animals.
For protein antigens >10 kDa: 5 mg of protein should be provided for hen and rabbit antibodies, 2.5 mg for guinea pig and rat antibodies and 2 mg for mouse antibodies (solubilized or lyophilized). A minimum concentration of 1 mg/ml is required. Buffered NaCl solutions are preferred. Please dialyze out any urea, imidazole or sodium azide.
Final ELISA are performed on your antisera and pre-immune sera for individual animals and we guarantee a minimum good titer for all your projects. A detailed ELISA report is supplied with every project and complete instruction and reagents used are described so you can reproduce the analyses.