Monoclonal Antibodies

 

Type Host Hapten Service features Delivery Lead time

Anti-protein monoclonal antibodies

(you supply your protein antigen)

Mouse Customer provides:
• Purified protein
• >90% purity
• >10 KDa
• Minimum amount:   5 mg
Immunisation and sera collection
Titer evaluation (ELISA)
Fusion and hybridoma development
ELISA screening and cloning
Isotype determination
Ascites production and purification (2 clones)
Approx. 2 mg purified antibodies
ELISA result - Titer guaranteed >1:50:000
Isotype determination result
Project report
23-26 weeks

Anti-peptide monoclonal antibodies

(we synthesize the peptide)

 

Mouse We prepare synthetic peptide
12-20 aa, with terminal cys for conjugation
Immunogen: KLH-peptide conjugate
Target protein analysis and peptide design
Synthesis, purification & conjugation of your peptide (12-20 aa)
Immunisation and sera collection
Best titer by ELISA
Fusion and hybridoma development
ELISA screening and cloning
Isotype determination
Ascites production and purification (2 clones)
Approx. 2 mg purified antibodies
ELISA result - Titer guaranteed >1:50:000
Isotype determination result
Project report
2-3 mg free peptide, purity >90%
LC & MS analysis
24-28 weeks

 

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