Yeast is a very economical Eukaroytic expression system that presents many post-translational modifications yielding functional proteins. Because protein biological activity is highly dependent upon proper folding and specific glycosylations as well as other modifications (phosphorylation, acetylation, etc), yeast expression system may be a beneficial alternative to bacterial expression.
Genosphere Biotechnologies’ team will express your protein either as secreted heterologous protein or through intracellular expression.
All services include multiple steps from gene to purified protein as described in the table below.
The production of a protein takes about approximately 10-12 weeks.
|Description||Resulting Material||Average turnaround (*)|
|Gene Synthesis Stage|
-Cloning in standard pUC plasmid
|Cloned, synthesized and verified gene||1-2 weeks|
|Subcloning in Expression Vector Stage|
-Subcloning in expression vector
|Expression vector with cloned target gene||2-3 weeks|
|Pilot Expression Stage|
|-Expression vector prep
-Transformation using 3 different strains
-Small scale expression and optimization experiments
-Determine yields and protein behavior
|10-50 µg of semi-purified protein||2-3 weeks|
|Expression and Purification Stage|
|-Scale up culture
-SDS-PAGE, WESTERN blot
| 0.5-5 mg of purified protein
Purity >80% or >90%
(*) Estimated production time for project of average difficulty. Actual turn around time varies with individual project features.