Custom Services
For Research
Custom Genes
Cloning Vectors
Cloning Vectors
► Standard free cloning vectors
We include free routine cloning into our vector pGB219 or alternatively into pUC57 high copy E. coli plasmid with any gene synthesis order.
Thus, you may choose among the standard vectors shown in the table below, for simple ORF with no toxicity.
| Free cloning vectors | Selection | Sequence information | Vector description |
|---|---|---|---|
|
pGB219
|
Ampicillin
(10-20 µg/ml)
|
MCS |
• 2716 bp
• Circular
• High copy plasmid
|
|
pUC57-Amp
|
Ampicillin
(10-20 µg/ml)
|
GenBank No. Y14837.1 |
• 2710 bp
• Circular
• High copy plasmid
|
|
pUC57-Kan
|
Kanamycin
(100 µg/ml)
|
GenBank No. JF826242.2 |
• 2665 bp
• Circular
• High copy plasmid
|
pGB219 is a pUC19 vector with modified restriction site MCS region. All our genes are normally cloned into our vector. It is high copy plasmid, with ampicillin resistance marker and white/blue LacZ expression.
►Custom cloning into your vector
Alternatively, we can clone your gene into any vector of your choice.
You would need to provide us with:
- 5 ug of purified plasmid vector
- Sequence information
Additional charge applies, please request a quote.