Mammalian cells are excellent hosts for the production of complex recombinant proteins that require extensive folding, the assembly of subunits and/or posttranslational modifications. For these reasons, mammalian cells are widely used by the biotech industry for the production of bio-active proteins.
Different mammalian cell types are used according to the quantity and quality of product required.
All services include multiple steps from gene to purified protein as described in the table below.
Leadtime is approximately 10-14 weeks.
|Description||Resulting Material||Average turnaround (*)|
|Gene Synthesis Stage|
-Cloning in standard pUC plasmid
|Cloned, synthesized and verified gene||1-2 weeks|
|Subcloning in Baculovirus Transfer Vector Stage|
-Subcloning Bac-donor vector
|Plasmid vector with cloned target gene||2 weeks|
|Bacmid Generation Stage|
-Recombinant bacmid selection
-Recombinant bacmid DNA isolation
|Bacmid DNA with cloned target gene||2 weeks|
|Insect Cells Transfection Stage|
|-Multiple transfection experiments
-SDS-PAGE / Western blot analysis
|High titer recombinant baculovirus stock||3 weeks|
|Pilot Expression Stage|
|-Expression pilot and optimization experiments
-Determine yields and protein behavior
|10-50 µg of semi-purified protein||1-2 weeks|
|Expression and Purification Stage|
-SDS-PAGE, WESTERN blot
| 0.1 to 1 mg of purified protein
(*) Average production time for average difficult project. Actual turn around time varies with individual project features.