Wir bieten die kostengünstige Synthese von “custom peptide libraries” im 96-wells Mikroplattenformat an. Durch die Nutzung einer simultanen Multi-Peptid Synthesetechnologie, sind wir in der Lage auch ihre Bedürfnisse zeitnah erfüllen zu können. Folgende Anwendungsgebiete für „Custom peptide libraries“ sind in der Literatur u.a. beschrieben worden: Peptidscreening für das “enzyme substrate profiling”, “protein-protein interaction region mapping”,” antibody epitope mapping”.
Peptidlänge: 5-18 AS
Freie C-und N- termini, ungebundene Peptide
Menge: 1 mg (sequenzabhängig)
Reinheit: Screening / „Crude“ , durchschnittliche Reinheit 12mer >70%
Analysedaten: Massenspektrum für jedes Peptid
Lieferzeit: durchschnittlich 2-3 Wochen
Many synthetic library design have been successfully developed for various applications.
Mutational peptide library
Mutational peptide library design involves The synthesis of peptides with single or multiple point mutations. This approach is useful for understanding the effect of mutations on the activity of a peptide, as well as for designing peptides with desired activities.
Truncation peptide library
Truncation peptide library design involves synthesising peptides with truncated sequences. This approach is used to identify shorter peptides with the same activity as longer peptides. It is also useful in determining the minimum sequence required to produce a certain activity, or the minimum size of a peptide necessary for a certain function.
Cyclic peptide library
Cyclic peptide library design involves synthesising peptides that are cyclic in structure. This approach is useful for understanding the role of the cyclic structure of a peptide in its activity, as well as for designing peptides with desired activities.
Overlapping peptide library
Overlapping peptide libraries are constructed by breaking down a protein or peptide sequence into multiple short peptides that overlap with each other. The length and offset of these peptides are determined in advance and are typically set to be 8 to 20 amino acids in length, with an offset of 2-4 amino acids.
This approach provides comprehensive coverage of the target protein and enables the identification of specific peptides that bind to the target molecule through techniques such as mass spectrometry or protein-protein interaction analysis. The optimal length and offset for epitope mapping have been determined to be within the aforementioned range.
In vitro evolution peptide library
In vitro evolution library design involves creating a library of peptides by subjecting them to in vitro selection and amplification. This method is useful for discovering new peptides with desired activities, or for engineering existing peptides to have specific activities. The library is created by subjecting peptides to selection criteria, such as binding to a target molecule, and then amplifying the successful peptides to generate a large library. This approach is often used in combination with other methods, such as random library design and recombinant library design.
Alanine scanning peptide library
Alanine scanning peptide library design involves replacing a single amino acid residue in a peptide with alanine, and then synthesising a library of peptides where each one contains a different alanine substitution. This approach is useful for determining which amino acid residues are involved in the activity of a peptide, as well as what the relative importance of each residue is.
Positional scanning peptide library
Positional Scanning Library is a key method for peptide sequence optimization. By sequentially replacing selected amino acid residues with all other natural amino acids, it has the ability to identify potentially more favorable residues at specified positions for improved peptide activity.