Antibodies Purification

Following polyclonal antibodies production as described above, you may wish to purify your antiserum by chromatography. Genosphere Biotechnologies offers two standard purification services.

 

Total IgG purification

Total IgG purification service on Protein A column is recommended for improving signal to noise ratio by eliminating all irrelevant serum proteins and macromolecules. This service includes:

 

  • Affinity chromatography of your antiserum
  • Analysis of collected fractions
  • ELISA

Purification of total IgG from the serum results in a highly pure (>90%) and stabilized polyclonal antibodies preparation.

 

Affinity purification

The second purification service includes affinity chromatography on antigen-bound or peptide-bound support. This approach is recommended when specific IgG are needed in your application, or if further antibodies labeling is intended.

 

This service includes:

  • Preparation of the affinity chromatographic support by covalently linking of your antigen(*) or your peptide
  • Affinity chromatography of your antiserum
  • Analysis of collected fractions
  • ELISA

(*) An additional supply of 5 mg of antigen is required for this service.

Fig 6. (a) Affinity chromatography typical elution profile.

Click on figure to enlarge
Fig 6. (b)
ELISA responses of the various fractions for a 15aa anti-peptide antisera.
Click on figure to enlarge
Fig 6. ( c ) SDS PAGE of a typical affinity-purified antibody.

Click on figure to enlarge

Navigation