Fig. 9. Stem-loop to random coil thermal transition profile of a Fluorescein- Dabcyl 35-mer Molecular Beacon. The stem sequence is indicated. A 250- nM solution of the probe in 100 mM Tris (7.5), 1 mM MgCl2 was heated from 22°C to 77°C in 5°C increments and Fluorescence recorded (Ex 490nm/ Em 520 nm).
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