- Fig 7. ELISA response of the phospho-specific antibodies against (a) the phosphorylated sequence and (b) the non-phosphorylated sequence.
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This service allows the production of antibodies that will specifically recognize the phosphorylated state of your target protein. The approach includes the synthesis and purification of phosphorylated and non-phosphorylated peptides, immunisation, fabrication of two affinity columns for successive purification. Extensive ELISA on phospho-specific antibodies as well as on the fraction specific to the non-phosphorylated state is performed. We have successfully utilized this method with other post-translational modifications including acetylation and methylation.