custom services for research
Protein Expression
E. coli Expression System
Escherichia coli (E. coli) is one of the most widely used hosts for the heterologous protein expression. Its genetics are far better characterized than those of any other microorganism.
Guaranteed Expression Packages
ref. ECOLI01 : 5 mg of purified >95%, soluble protein
ref. ECOLI02 : 15 mg of purified >95%, soluble protein
Expression services description
All services include multiple steps from gene to purified protein as described in the table below.
| Description | Resulting Material | Average turnaround (*) |
| Gene Synthesis Stage | -Codon optimization -Gene synthesis -Cloning in standard pGB219 plasmid -Complete sequencing |
Cloned, synthesized and verified gene | 1-2 weeks |
| Subcloning in Expression Vector Stage | -Plasmid prep -Subcloning in expression vector -Clone selection -Complete sequencing |
Expression vector with cloned target gene | 1-2 weeks |
| Pilot Expression Stage | -Expression vector prep -Transformation using 3 different strains -Protein induction and optimzation experiments -Determine yields and protein behavior |
10-50 µg of semi-purified protein | 1-2 weeks |
| Expression and Purification Stage | -Expression of 1 to 5 L bacterial culture -Protein extraction -Protein purification -SDS-PAGE, WESTERN blot |
5-20 mg of purified protein Purity >95% |
2-3 weeks |