Figure 5. WESTERN blot analysis is performed for every project. Semi-purified protein antigen was provided as shown on the coomassie-stained polyacrylamide gel. We further purified the the 48 kDa protein of interest to > 90% purity and used it to raise polyclonal antibodies in guinea pig. The resulting antiserum was diluted 1/2000 for WESTERN blot analysis of the provided protein extract. The blot reveals a single band as expected.

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Standard offer
This service includes the following features:

• Immunization of 2 animals with your antigen
  • Rabbits: 100 ml of ELISA titered antiserum
  • Guinea pigs: 20 ml of ELISA titered antiserum
• Pre-immune control serum
• ELISA data
• WESTERN blot analysis

Provided antigen samples
Antigens may be supplied in solution, lyophilized, as gel slice, inclusion bodies, insoluble aggregates, GST/His fusions, protein conjugates, etc. It should be noted that small proteins (<10 kDa), peptides, organic molecules need to be conjugated to a carrier protein in order to elicit a good immune response in animals.

Sample requirements
For protein antigens >10 kDa: 5 mg of protein should be provided for hen and rabbit antibodies and 2 mg for guinea pigs (solubilized or lyophilized). A minimum concentration of 1 mg/ml is required. Buffered NaCl solutions are preferred. Please dialyze out any urea or imidazole.

Quality control
Final ELISA are performed on your antisera and pre-immune sera for individual animals and we guarantee a minimum good titer for all your projects. A detailed ELISA report is supplied with every project and complete instruction and reagents used are described so you can reproduce the analyses. In addition, depending upon quality and quantity of antigen supplied, we control your antibodies by WESTERN blotting ensuring perfect specificity (Figure 5).